Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 648091..648818 Replicon chromosome
Accession NZ_CP060963
Organism Escherichia coli strain EC7

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag H9194_RS03165 Protein ID WP_000550189.1
Coordinates 648091..648405 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag H9194_RS03170 Protein ID WP_000560266.1
Coordinates 648402..648818 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9194_RS03145 (644258) 644258..645244 - 987 WP_023281494.1 Gfo/Idh/MocA family oxidoreductase -
H9194_RS03150 (645323) 645323..646006 - 684 WP_001183042.1 vancomycin high temperature exclusion protein -
H9194_RS03155 (646083) 646083..646586 - 504 WP_001295542.1 M48 family metallopeptidase -
H9194_RS03160 (646671) 646671..647807 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
H9194_RS03165 (648091) 648091..648405 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
H9194_RS03170 (648402) 648402..648818 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
H9194_RS03175 (648863) 648863..650881 - 2019 WP_241462803.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
H9194_RS03180 (651307) 651307..653658 - 2352 WP_024238710.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T171746 WP_000550189.1 NZ_CP060963:648091-648405 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T171746 NZ_CP078087:c1436128-1435850 [Stutzerimonas stutzeri]
ATGATCAAAACGTTCCAGCACAAAGGGCTTCGCAAGCTCTTCGAAACCGGCAGCACGGCCGGCGTACAGCCAGCCCATGC
CAAGCGGCTACGGATGCAACTGGCGGCCCTGGACACGGCCATGACAATAGACGACATGGATATCCCCGGCTTCCGGCTGC
ACCCGCTCAAGGGCGAAATGCGGGGGCGCTGGTCGATCATGGTGAACGGCAACTGGCGGCTGACGTTCGAGTTTCGCGAC
GGAAACGCTTATGTCCTGGACTATGAGGATTACCACTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT171746 WP_000560266.1 NZ_CP060963:648402-648818 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT171746 NZ_CP078087:c1435850-1435563 [Stutzerimonas stutzeri]
ATGAACATGCACAACCCTCCCCATCCTGGTGAGTTCATCACCGATATCTACCTGGAGCCGAACGGCATCAGCGGGCGTGA
GCTGGCGGAAAAGCTCGGTGTTGCACCCTCTACCCTGAGCCGCGTCTTGAAGGGCACCAGTCGCGTTACGCCGGAAATGG
CATTGCGTCTGTCCGTAGCGCTTGGCCGCTCGCCCGAGAGCTGGCTGGCCATGCAGGATGCCTACGATCTGTGGGTTGCC
CGGCAACATGTGGATCTGCAGCATGTTGGCAAACTGGCTTTTGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References