Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 604766..605565 Replicon chromosome
Accession NZ_CP060963
Organism Escherichia coli strain EC7

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag H9194_RS02940 Protein ID WP_000347273.1
Coordinates 604766..605230 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag H9194_RS02945 Protein ID WP_001307405.1
Coordinates 605230..605565 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9194_RS02910 (599767) 599767..600201 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
H9194_RS02915 (600219) 600219..601097 - 879 WP_001300474.1 PTS N-acetylgalactosamine transporter subunit IID -
H9194_RS02920 (601087) 601087..601866 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
H9194_RS02925 (601877) 601877..602350 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
H9194_RS02930 (602373) 602373..603653 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
H9194_RS02935 (603902) 603902..604711 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
H9194_RS02940 (604766) 604766..605230 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
H9194_RS02945 (605230) 605230..605565 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
H9194_RS02950 (605714) 605714..607285 - 1572 WP_001723936.1 galactarate dehydratase -
H9194_RS02955 (607660) 607660..608994 + 1335 WP_001723935.1 galactarate/glucarate/glycerate transporter GarP -
H9194_RS02960 (609010) 609010..609780 + 771 WP_001723934.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T171745 WP_000347273.1 NZ_CP060963:c605230-604766 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T171745 NZ_CP078087:1280698-1281012 [Stutzerimonas stutzeri]
ATGATCTTTATCGAGACACCGATCTTTACCAAGCGCCTGCGGGATCTGCTTAGCGATGACAGCTACGCGGAGTTTCAGCG
GCAACTGGCTGACCGGCCGGACATGGGCGATGTGATTGAAGGTACTGGAGGCATTCGCAAGGTTCGCGTTGCGTCTGGCG
GTCACGGTAAGCGAGGCGGGTCCAGGGTCATCTACTACCACTTCACGGCGGCTTCGCAGATCGCATTACTGCTGATCTAT
CCGAAGAACGAGAAGGACGACCTGACGGCAGATGAGCGCAAGGTGCTCAAGCAAATCATCGAGCGGTGGAGGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT171745 WP_001307405.1 NZ_CP060963:c605565-605230 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT171745 NZ_CP078087:1281018-1281308 [Stutzerimonas stutzeri]
ATGAGCAAATTCTTCGAAGACCTTCTAGAAAGCGTCCAGCAGATGGACGAGATTCACCGTGGCGAACGTCAACCCTCGCG
GGAATTCGTCGTCGACTCGCTGCAGGTGAAAGAGATCCGCAAGGCGACCGGTCTGACCCAGGCCAAGTTCGCGGCGATGA
TCGACGTTCAACTGGGTACGCTGCGTAACTGGGAACAAGGCCGACGCGAACCGACCGGACCCGCAAAAGCGCTGCTGCGC
GCCATCCATAACGATCCGAAGAACGTCATCCAAGCGCTTTCTTCGGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References