171742

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 4169886..4170565 Replicon chromosome
Accession NZ_CP060953
Organism Escherichia coli strain EC8

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag H9195_RS20110 Protein ID WP_000057523.1
Coordinates 4169886..4170188 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag H9195_RS20115 Protein ID WP_000806442.1
Coordinates 4170224..4170565 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9195_RS20090 (4165149) 4165149..4166369 - 1221 WP_001773866.1 fosmidomycin MFS transporter -
H9195_RS20095 (4166587) 4166587..4168239 + 1653 WP_001773867.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
H9195_RS20100 (4168276) 4168276..4168755 - 480 WP_001538397.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
H9195_RS20105 (4168959) 4168959..4169753 - 795 WP_001538398.1 TraB/GumN family protein -
H9195_RS20110 (4169886) 4169886..4170188 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H9195_RS20115 (4170224) 4170224..4170565 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
H9195_RS20120 (4170623) 4170623..4173127 - 2505 WP_000083948.1 copper-exporting P-type ATPase CopA -
H9195_RS20125 (4173389) 4173389..4174321 + 933 WP_001538399.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T171742 WP_000057523.1 NZ_CP060953:4169886-4170188 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T171742 NZ_CP078071:c2834601-2834263 [Burkholderia sp. EMB26]
ATGGTTGACACATTGCAATGCATGCCCTACAGTCCTCCTTCATTCGGCATCCGGACCATCGACGTCTTCGACGCCTGGTT
TGCCGGCCTGCCCGATCGCCTCGCCAAACGCCGCATCCAGGCGCGCATCGACCGCCTGTCGATGGGCAATCCGGGCAACT
GGAAATCCGCCGGCTCCCCCGTCGTCGAAATGCGTATCGACCACGGCCCCGGCTATCGCGTCTATGACGTGAGGCGCGGA
ACGATATGGGTCATCCTGCTGTGCGGCGGCGATCAATCGACGCAACAGGCCGACATCCGCGCCGCGCACGCAATGCTCGC
GCACCTCGACATGGAGTGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT171742 WP_000806442.1 NZ_CP060953:4170224-4170565 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT171742 NZ_CP078071:c2834259-2833960 [Burkholderia sp. EMB26]
ATGGACAAGATCAAGACGCGGCCGTGGGATTCGGCCGAACACCTGAAAACCGAAGACGACATGGCCGACTACTTCGACGC
ATGCCTGCAGGAGGCCGGCGACGATCCGGCCTTCATCGCGCATGCGCTCGGCGTGATCGCCCGCGCACGCGGCATGTCGC
AGGTCGCACGCGATGCGGGCCTGTCGCGCGAAGGGCTGTACAAGGCGCTGTCGCACGACGGCAATCCGAGCTTCGGCACG
ATCCTGAAAGTCATCAAGGCGCTCGGCCTGCAATTGCACGGCGCCAAGGCTGCCGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
AlphaFold DB S1QAY3

References