Detailed information of TA system TA171733

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-agrB/SymE(toxin)
• Location: 3550931..3551343
• Replicon: chromosome
• Accession: NZ_CP060953
• Organism: Escherichia coli strain EC8

Toxin (Protein)

• Gene name: symE
• Uniprot ID: S1NWQ7
• Locus tag: H9195_RS17185
• Protein ID: WP_000132614.1
• Coordinates: 3550931..3551272 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	agrB
Locus tag	-
Coordinates	3551267..3551343 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9195_RS17160 (3546000)	3546000..3547280	-	1281	WP_001338077.1	DUF445 domain-containing protein	-
H9195_RS17165 (3547465)	3547465..3548385	+	921	WP_001513532.1	Rpn family recombination-promoting nuclease/putative transposase	-
H9195_RS17170 (3548613)	3548613..3548693	+	81	WP_020233658.1	hypothetical protein	-
H9195_RS17175 (3548791)	3548791..3549667	+	877	Protein_3360	DUF262 domain-containing protein	-
H9195_RS17180 (3549721)	3549721..3550884	+	1164	WP_224153787.1	DUF1524 domain-containing protein	-
H9195_RS17185 (3550931)	3550931..3551272	-	342	WP_000132614.1	endoribonuclease SymE	Toxin
- (3551267)	3551267..3551343	+	77	NuclAT_8	-	Antitoxin
- (3551267)	3551267..3551343	+	77	NuclAT_8	-	Antitoxin
- (3551267)	3551267..3551343	+	77	NuclAT_8	-	Antitoxin
- (3551267)	3551267..3551343	+	77	NuclAT_8	-	Antitoxin
- (3551267)	3551267..3551343	+	77	NuclAT_9	-	Antitoxin
- (3551267)	3551267..3551343	+	77	NuclAT_9	-	Antitoxin
- (3551267)	3551267..3551343	+	77	NuclAT_9	-	Antitoxin
- (3551267)	3551267..3551343	+	77	NuclAT_9	-	Antitoxin
H9195_RS17190 (3551493)	3551493..3553262	-	1770	WP_001513535.1	restriction endonuclease subunit S	-
H9195_RS17195 (3553262)	3553262..3554731	-	1470	WP_001387312.1	type I restriction-modification system subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12266.07 Da
• Isoelectric Point: 8.4982

>T171733 WP_000132614.1 NZ_CP060953:c3551272-3550931 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T171733 NZ_CP078069:1631976-1632563 [Comamonas sp. NLF-1-9]
ATGAGTTCGCACTTCACCGTCGTCTATGACGCCTGCGTGCTCTATCCGGCACCGCTGCGCGATTTGCTGATGCATCTGGC
GCTGTCGGATCTGTACCGGGCGCGCTGGAGCGACATGCTCCACGACGAGTGGACGCGCAATGTGCTGGCCAGCCGCCCCG
ACTTGACCCAAGAGCAGCTGAACCGGACGCGCCAGTTGATGAATGCTCATGTCCGGGACAGTCTGGTCACCGGCTTCGAG
TACTTGATTCCATCAATCAAGCTGCCCGATCCGGATGACCGCCACGTGGTGGCCGCCGCCATCCACTCCGGGGCCAGCCT
GATCGTGACCTTCAACCTCAAGGACTTCCCGTTCGACGCGCTCAAGCCCTGCAACCTCGCCGCGCAGCATCCAGACGACT
TCATCGTTGATCTGCTGGATCTGCACCCCGCACCGGCGAGCGCATCCGCGACAAGATCGCCGCCAGCAAACGCAAAGGGA
TGTGGATGGGCGGCGTACCACCCCTGGGCTACGACGTCGAGAACCGCCTGCTGGTCATCAACGAAACCGAGGCTGCGGTG
GTGCGGCGCATCTTCGAGGAGATGCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT171733 NZ_CP060953:3551267-3551343 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: S1NWQ7

Antitoxin