Detailed information of TA system TA171729

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2945460..2946062
• Replicon: chromosome
• Accession: NZ_CP060953
• Organism: Escherichia coli strain EC8

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: H9195_RS14285
• Protein ID: WP_000897302.1
• Coordinates: 2945460..2945771 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H9195_RS14290
• Protein ID: WP_000356397.1
• Coordinates: 2945772..2946062 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9195_RS14255 (2940490)	2940490..2941275	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
H9195_RS14260 (2941374)	2941374..2941973	+	600	WP_001314324.1	glucose-1-phosphatase	-
H9195_RS14265 (2941967)	2941967..2942839	+	873	WP_000920762.1	virulence factor BrkB family protein	-
H9195_RS14270 (2942836)	2942836..2943273	+	438	WP_000560978.1	D-aminoacyl-tRNA deacylase	-
H9195_RS14275 (2943318)	2943318..2944259	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
H9195_RS14280 (2944323)	2944323..2945231	-	909	WP_001774092.1	alpha/beta hydrolase	-
H9195_RS14285 (2945460)	2945460..2945771	+	312	WP_000897302.1	hypothetical protein	Toxin
H9195_RS14290 (2945772)	2945772..2946062	+	291	WP_000356397.1	NadS family protein	Antitoxin
H9195_RS14295 (2946147)	2946147..2946359	+	213	WP_000197774.1	hypothetical protein	-
H9195_RS14300 (2946421)	2946421..2946699	+	279	WP_001296612.1	hypothetical protein	-
H9195_RS14305 (2947095)	2947095..2947313	+	219	WP_001314326.1	CopG family transcriptional regulator	-
H9195_RS14310 (2947529)	2947529..2948458	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
H9195_RS14315 (2948455)	2948455..2949090	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
H9195_RS14320 (2949087)	2949087..2949989	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T171729 WP_000897302.1 NZ_CP060953:2945460-2945771 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T171729 NZ_CP078069:529645-529791 [Comamonas sp. NLF-1-9]
ATGTTCTACAAAAGCATGACGACCCATGCCGACCATACCCTGTGGCAAGACGTGTACCACGCACCTTGTCCCAATGGCTG
CATGGCCTACATCAAAGTCACCTTTCGCGCGGATGGCGCGGTGGTGCTCCAGTTCAAGGAGCTTTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT171729 WP_000356397.1 NZ_CP060953:2945772-2946062 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT171729 NZ_CP078069:529793-530212 [Comamonas sp. NLF-1-9]
ATGACCCCCGACGCAAAGAACCGGTACTGCCTGCAATGCGACGACGGCACGGCCCTGGTGCACACCACCAAGGACGTGCA
TGGCGAGATCATGGGCGTGCCCTATTCCGTGAAGGGCGTCACTGGCTGGCATTGCCCGGCCTGCGGCGAAATCGAATACG
ACTTGCAGGGCGACAGCGCCGCCCGCATGAGCGCGGCCGTGGAGGCCGCTGGCCAGGAGGCCAGGCAACGGCAAGCCCTG
GCGCTGCGCGCCGCGCGCAAAAAACTGGGCTTGTCGCAAGCCGAGGCGGGACGTCTGTTTGGCGGCGGTGTATCGGCTTT
CAGCGAATATGAACGCGGCAAGACCCAGCCACACAAGTCCACCGTGCTGCTGATGCGCTTGCTGGACAAGCACCCCAACC
TGCTGGCCGAGGTGCGATAG

Structures

Toxin

• AlphaFold DB: S1P416