171727

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2059469..2060196 Replicon chromosome
Accession NZ_CP060953
Organism Escherichia coli strain EC8

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag H9195_RS10005 Protein ID WP_000550189.1
Coordinates 2059882..2060196 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag H9195_RS10000 Protein ID WP_000560266.1
Coordinates 2059469..2059885 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9195_RS09990 (2054629) 2054629..2056980 + 2352 WP_000695487.1 alpha-glucosidase -
H9195_RS09995 (2057406) 2057406..2059424 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
H9195_RS10000 (2059469) 2059469..2059885 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
H9195_RS10005 (2059882) 2059882..2060196 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
H9195_RS10010 (2060480) 2060480..2061616 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
H9195_RS10015 (2061701) 2061701..2062204 + 504 WP_001333820.1 M48 family metallopeptidase -
H9195_RS10020 (2062281) 2062281..2062973 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
H9195_RS10025 (2063052) 2063052..2064038 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T171727 WP_000550189.1 NZ_CP060953:c2060196-2059882 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T171727 NZ_CP078069:c457333-457022 [Comamonas sp. NLF-1-9]
ATGAAGAAGCCCGAAAAAATCAAGATCTCTGACCTGCCCGTGTTCGATGCTGCGGAGTACCTGAAGAGCGAAGAGGACAT
TGCCATTTACCTCACGCTGGCGATTGAAGAGAACGACCCTGCGGGCCTGGCTGATGCGCTGGGCACAATTGCACGCGCTC
GCGGCATGACCGAGGTGGCGCGGGCTAGCGGGCTGACCCGCGAGGCGCTTTACAAGGCCCTGCGCCCCGGCAGCCATCCG
CGCTTTGACACCATCGCCCGCGTGTGTGGAGCGTTGGGCGTGAAGCTGGTGGCGCAGCCAAGCCGTACTTAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT171727 WP_000560266.1 NZ_CP060953:c2059885-2059469 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT171727 NZ_CP078069:c457633-457337 [Comamonas sp. NLF-1-9]
ATGTACACGATTCAGCGCACGGAACAGTTTTCTGGCTGGCTGGATGGCTTGAAAGACCGGGTGGCGCGAGGTCGCCTTGT
TCGTCGACTGGAGAAGGCGCAGCGCGGGCTGCTGGGTGACGTTCAGCATGTAGGCGAAGGCGTGTTTGAGATGCGCGAGT
TCTTTGGCCCTGGCTGGCGCATGTACTACGTTCAGCGCGGCGCGGTGTTGATTGTGATGCTGGGCGGGGGCGACAAGTCC
ACCCAGCAGGCTGACATTGCTGCCGCGCAGACTTTGGCTGCAACCCTTGAGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References