Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 821878..822380 Replicon chromosome
Accession NZ_CP060953
Organism Escherichia coli strain EC8

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag H9195_RS04240 Protein ID WP_000767822.1
Coordinates 821878..822132 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag H9195_RS04245 Protein ID WP_001259255.1
Coordinates 822129..822380 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9195_RS04220 (818371) 818371..819729 - 1359 WP_000019194.1 putrescine/proton symporter PlaP -
H9195_RS04225 (819719) 819719..819781 - 63 WP_010723108.1 membrane protein YoeI -
H9195_RS04230 (819996) 819996..820925 - 930 WP_000803366.1 LysR substrate-binding domain-containing protein -
H9195_RS04235 (820971) 820971..821795 - 825 WP_000754737.1 SDR family oxidoreductase -
H9195_RS04240 (821878) 821878..822132 - 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
H9195_RS04245 (822129) 822129..822380 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
H9195_RS04250 (822663) 822663..822713 + 51 WP_001364200.1 his operon leader peptide -
H9195_RS04255 (822859) 822859..823758 + 900 WP_000131779.1 ATP phosphoribosyltransferase -
H9195_RS04260 (823764) 823764..825068 + 1305 WP_001445483.1 histidinol dehydrogenase -
H9195_RS04265 (825065) 825065..826135 + 1071 WP_001538918.1 histidinol-phosphate transaminase -
H9195_RS04270 (826135) 826135..827202 + 1068 WP_001538920.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T171723 WP_000767822.1 NZ_CP060953:c822132-821878 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T171723 NZ_CP078066:c4656586-4656149 [Bradyrhizobium arachidis]
GTGTACCTCGTCGATACCAACGTCATATCCGCGGTCTCGCCGAACCGGCTGGCGCCCGCGGCGCTGGTCGAATGGATGGA
TGCGCACTCCGCTTCGTTGTTCTTGTCTGCGGTGACGATTGCGGAGGTCGAGGACGGAATCGCCAAGCTGCGGCGCGAGA
AGGCCACGCGCAGGAGCAAGGACTTGGCCCAATGGCTAGACGTCGTATTGCATCTCTATGGCGACCGAATTCTGGCGTTC
GATACGGCGGTCGCGCGGCTCGCGGGAGAGCTATCGGACCGGGCTCGCGGGCAGGGGCATGCGCCCGGATTTGCCGATAT
CATCATTGCCGCCACGGCGCGTCAGCACGCGCTGACGATCCTGTCGCGCAATGTCAGGCACTTCGAGCCGCTGGGTGTGC
CAGTGCTCGATCCCTTTAGTGAGTTGCCGCCGTCCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT171723 WP_001259255.1 NZ_CP060953:c822380-822129 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT171723 NZ_CP078066:c4656832-4656590 [Bradyrhizobium arachidis]
ATGCGAGAAATCCAGCTTCGTGATGCCAAGGCCAATCTCTCTGCAGTCGTCGATGAAGCCATTGACGGCAAGCCGGCGGT
GATCACGCGCCATGGCAAGAAGCAGGCGGTGGTCCTGAGCTACGAGGAGTGGCGCCGGCTTTCGCAGGTCCCGAGCTTCG
GACGTCTTTTGATGGCTGCTCCCGCGAGGGCGGGGGATCTGCCGGCGCGCAATCGGTCCAAGCTACGCAAGACCGATTTC
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References