Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 213197..213759 Replicon chromosome
Accession NZ_CP060953
Organism Escherichia coli strain EC8

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag H9195_RS01010 Protein ID WP_000605675.1
Coordinates 213481..213759 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag H9195_RS01005 Protein ID WP_000781370.1
Coordinates 213197..213481 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9195_RS00990 (208378) 208378..211425 + 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -
H9195_RS00995 (211438) 211438..212322 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
H9195_RS01000 (212315) 212315..212968 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
H9195_RS01005 (213197) 213197..213481 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
H9195_RS01010 (213481) 213481..213759 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H9195_RS01015 (213945) 213945..214955 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
H9195_RS01020 (215089) 215089..216786 - 1698 WP_000433460.1 malate dehydrogenase -
H9195_RS01025 (216943) 216943..217080 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
H9195_RS01030 (217182) 217182..217397 - 216 WP_000495766.1 biofilm-dependent modulation protein -
H9195_RS01035 (217742) 217742..218173 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T171714 WP_000605675.1 NZ_CP060953:c213759-213481 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T171714 NZ_CP078038:2704573-2704866 [Shewanella putrefaciens]
ATGCCACAAATAGTCTTCACTGCCACTGCATTACGTGATTTAGAACGCTTACGTGAGTTTCTTCGCAGTAAAAAACCCCC
TGCCGCACAACGGGCTGCCAGCGCCATAATCAATACCATTAGAAAACTGGAAAGCTATCCAGACATTGGTCGGCCTATCG
ATGACAATGACTTTAGCTTTAGGGAACTGCTCATCGACTTTGGTGATACGGGTTATCTGGCAATGTACCAGTATGATGGC
GGCGAACGATTGACCGTATTATGTATCCGTCACCAAAAAGAAGCCGGATACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT171714 WP_000781370.1 NZ_CP060953:c213481-213197 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT171714 NZ_CP078038:2704301-2704585 [Shewanella putrefaciens]
ATGAGCACAATCAAACCCGTGTCGGTTAAATTAGATGCCGATATTAAAGCCAGAGTCGAGCATTTAGCCGAAACTCGTAA
ACGTTCATCACACTGGATGATGCGTGAAGCGATCCGTGAATATGTTGAGAGAGAAGAGAAACGCGAAGCTTTGCAGCAAG
AAGCGTTACGCGCATGGGAAGAACACCAGACATCAGGATTGCATGTCACCGGTGACGAAGTGGTGAATTGGCTGGAGTCC
TGGGGAAGTGAAAATGAAAAGGCTGCCCCTACATGCCACAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References