Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3291351..3292030 Replicon chromosome
Accession NZ_CP060950
Organism Escherichia coli strain EC9

Toxin (Protein)


Gene name higB Uniprot ID U9XN95
Locus tag H9196_RS15800 Protein ID WP_000057540.1
Coordinates 3291351..3291653 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag H9196_RS15805 Protein ID WP_000806442.1
Coordinates 3291689..3292030 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9196_RS15780 (3286614) 3286614..3287834 - 1221 WP_001251608.1 fosmidomycin MFS transporter -
H9196_RS15785 (3288052) 3288052..3289704 + 1653 WP_000771768.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
H9196_RS15790 (3289741) 3289741..3290220 - 480 WP_000186631.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
H9196_RS15795 (3290424) 3290424..3291218 - 795 WP_000365180.1 TraB/GumN family protein -
H9196_RS15800 (3291351) 3291351..3291653 + 303 WP_000057540.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H9196_RS15805 (3291689) 3291689..3292030 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
H9196_RS15810 (3292088) 3292088..3294592 - 2505 WP_023155691.1 copper-exporting P-type ATPase CopA -
H9196_RS15815 (3294854) 3294854..3295786 + 933 WP_023155692.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(7-100)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11781.35 Da        Isoelectric Point: 10.2638

>T171696 WP_000057540.1 NZ_CP060950:3291351-3291653 [Escherichia coli]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTSLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T171696 NZ_CP078030:c13251-12970 [Acinetobacter variabilis]
ATGGCTATTAAACTTCGCAAATGGGACAGTGCTGAGCATCTTAAAACAGAGGATGATATGCAGGCTTACTTACAAGCATG
TATTGAAGAATCTAATGGCGATGCCGCATTCATTGCAAAGGCTTTAGGTAACATTGCAAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGCTTAGGCCGAGAAAGTCTTTATAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAGCAATTTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT171696 WP_000806442.1 NZ_CP060950:3291689-3292030 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT171696 NZ_CP078030:c13554-13252 [Acinetobacter variabilis]
ATGTACTCAATATATACCACTGAAGTCTTTGATGACTGGTTCACCAAGTTAAAAGACCAGCAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGAAACTTTGGAGATACTGAGCCGGTCGGTGAAGGTGTTTCTGAATTACGTTTCT
TCTTTGGGCCAGGCTATAGGATTTATTACTGCAAGCAAGGGCAAAGGGTTGTTATTCTTTTAGCAGGCGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTGCAATTGGCACAAGATTTAGAGGAGGAGCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XN95

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References