Detailed information of TA system TA171690

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2138382..2138984
• Replicon: chromosome
• Accession: NZ_CP060950
• Organism: Escherichia coli strain EC9

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: H9196_RS10300
• Protein ID: WP_000897305.1
• Coordinates: 2138382..2138693 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H9196_RS10305
• Protein ID: WP_000356397.1
• Coordinates: 2138694..2138984 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9196_RS10270 (2133412)	2133412..2134197	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
H9196_RS10275 (2134296)	2134296..2134895	+	600	WP_001295269.1	glucose-1-phosphatase	-
H9196_RS10280 (2134889)	2134889..2135761	+	873	WP_000920762.1	virulence factor BrkB family protein	-
H9196_RS10285 (2135758)	2135758..2136195	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
H9196_RS10290 (2136240)	2136240..2137181	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
H9196_RS10295 (2137245)	2137245..2138153	-	909	WP_001162704.1	alpha/beta hydrolase	-
H9196_RS10300 (2138382)	2138382..2138693	+	312	WP_000897305.1	hypothetical protein	Toxin
H9196_RS10305 (2138694)	2138694..2138984	+	291	WP_000356397.1	NadS family protein	Antitoxin
H9196_RS10310 (2139570)	2139570..2139788	+	219	WP_001295676.1	CopG family transcriptional regulator	-
H9196_RS10315 (2140007)	2140007..2140249	+	243	WP_001086388.1	protein YiiF	-
H9196_RS10320 (2140579)	2140579..2141508	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
H9196_RS10325 (2141505)	2141505..2142140	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
H9196_RS10330 (2142137)	2142137..2143039	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T171690 WP_000897305.1 NZ_CP060950:2138382-2138693 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T171690 NZ_CP078028:25251-25532 [Acinetobacter variabilis]
ATGGCTATCAAACTTCGTAAATGGGACAGTGCTGAACATCTTAAAACAGAAGAAGATATGCAAGCTTACCTACAAGCCTG
TATAGATGAATCTAATGGTGATGCGGCATTCATTGCAAAAGCTTTAGGTAATATTGCTAAAGCTAAAGGAATGGCTCAAT
TATCAAGAGACACAGGTTTAGGCCGAGAAAGTCTTTATAAAGCACTTTCTGGCGATGTAAATCCAAGCTTTGATACTGTC
ATTAAAGTGGTAAAAGCACTGAATTTACGCTTAGCAATTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT171690 WP_000356397.1 NZ_CP060950:2138694-2138984 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT171690 NZ_CP078028:24948-25250 [Acinetobacter variabilis]
ATGTACTCAATATACACCACTGAAGTCTTTGATGACTGGTTTACTAAGTTAAAAGACCAGCAGGCAAAAAGGCGTATACA
AGTCCGAATTGATCGGGTTGAAGATGGAAACTTTGGAGATACTGAGCCAGTCGGTGAAGGTGTCTCTGAATTACGCTTCT
TCTTTGGACCTGGCTACAGGATCTATTACTGTAAGCAAGGGCAAAGGGTCGTTATTCTTTTAGCAGGCGGAGACAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTACAACTAGCACAAGATTTAGAGGAGGAGCTGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7