Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1347343..1348142 Replicon chromosome
Accession NZ_CP060950
Organism Escherichia coli strain EC9

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag H9196_RS06455 Protein ID WP_000347267.1
Coordinates 1347678..1348142 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag H9196_RS06450 Protein ID WP_001307405.1
Coordinates 1347343..1347678 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9196_RS06435 (1343129) 1343129..1343899 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
H9196_RS06440 (1343915) 1343915..1345249 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
H9196_RS06445 (1345624) 1345624..1347195 + 1572 WP_001273741.1 galactarate dehydratase -
H9196_RS06450 (1347343) 1347343..1347678 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
H9196_RS06455 (1347678) 1347678..1348142 + 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
H9196_RS06460 (1348197) 1348197..1349006 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
H9196_RS06465 (1349255) 1349255..1350535 + 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
H9196_RS06470 (1350558) 1350558..1351031 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
H9196_RS06475 (1351042) 1351042..1351821 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
H9196_RS06480 (1351811) 1351811..1352689 + 879 WP_001298758.1 PTS N-acetylgalactosamine transporter subunit IID -
H9196_RS06485 (1352707) 1352707..1353141 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1336688..1348142 11454


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T171688 WP_000347267.1 NZ_CP060950:1347678-1348142 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T171688 NZ_CP078027:557587-557973 [Acinetobacter variabilis]
ATGGATAATCGCTGTTTCAGGCTGAAACGCAGTAAGTGTGCGCTGGTGTTTCAGCTTTTTATTTTTGCTGTATTGATGTT
CTTGCTGTATCAGTTATTGCCTGTAATAATGTGGCTGGTTTGTGGCGTCATCGGTCTGGTCATCTACCAGTTGTTTTATC
GTAAGATACCGCACATTGAGCAGTTTGAATATCTGGATGGACGTGAATGGTCACTGACTGCAGCTGCACAAGGCACACGG
CGAGTGTTGATCAGTCACGTGATTGATCACCAAGCTTATATCGTGGTGTATTTCCAACATGCAAAAGCACGTCCGTTGCT
CATCTGGTGTGATCAGTTACCTTTTAAACAATGGAAGTCTTTGAAAGTTTTAACAAAAATACTTTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT171688 WP_001307405.1 NZ_CP060950:1347343-1347678 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT171688 NZ_CP078027:557337-557594 [Acinetobacter variabilis]
ATGACTGATGACATCACCTTAGAAGAGCGCAAAGTGATTTATCGCGCGCGTCGTGGCCTGAAAGAGATCGATGTATATTT
TGATCCCTACGTGAAAAACTATTATCTCAATGCTGATCCCTTCGAAAAAGAGATGTTTGCTGAGCTGGTTGCACAGGAAG
ATCCGGACCTGCTGGACTGGTTTATGGAAGTGTCAGAACCCCCACGCCCTGAATTAAAAGATTTTATCAAGAAGCTTAAA
TTTTACGTTCATGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References