Detailed information of TA system TA171682

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 85144..85397
• Replicon: plasmid pEC10-3
• Accession: NZ_CP060948
• Organism: Escherichia coli O157:H7 str. EC10

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: H9197_RS24020
• Protein ID: WP_001312851.1
• Coordinates: 85248..85397 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	85144..85203 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9197_RS23995 (81871)	81871..82617	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
H9197_RS24000 (82672)	82672..83232	+	561	WP_000139328.1	fertility inhibition protein FinO	-
H9197_RS24005 (83364)	83364..83564	+	201	WP_015059022.1	hypothetical protein	-
H9197_RS24010 (83950)	83950..84549	+	600	WP_032083981.1	PIN domain-containing protein	-
H9197_RS24015 (84611)	84611..84943	+	333	WP_152916585.1	hypothetical protein	-
- (85144)	85144..85203	-	60	NuclAT_1	-	Antitoxin
- (85144)	85144..85203	-	60	NuclAT_1	-	Antitoxin
- (85144)	85144..85203	-	60	NuclAT_1	-	Antitoxin
- (85144)	85144..85203	-	60	NuclAT_1	-	Antitoxin
H9197_RS24020 (85248)	85248..85397	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
H9197_RS24025 (85681)	85681..85929	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	fosA3 / mph(A)	-	1..86240	86240

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T171682 WP_001312851.1 NZ_CP060948:85248-85397 [Escherichia coli O157:H7 str. EC10]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T171682 NZ_CP078018:c2969822-2969427 [Acinetobacter junii]
ATGTACACGGCTAAACGTATTTTTGAGCTGCAATCTAGTCGATTTTCGCTAGGATTGCAGCTGTTTTGTTTTTTGAGTAT
CAATATCCTGCTTTATCAATTATTGTCAGCTTTTTTATGGTTAGTGTGCTTGTTGATTATGGGGTGGGCGGGCTGGTCGG
CATTAAACAAACCGAAAATAAATCAAATTGAGTATCTCTGTGACGATGATTGGTCGATTCGTTTTGATGGAGTAGAGCCT
AATATCGAGCAAGGAAAATTACTCAAAATTATTGATCATCATTTATATATTGTGCTGCATTTTTCTAATATTGAATCAAA
AACATGTGTAATTTGGTGGGATCAATTGTCTTTAAATCAATGGAAAATGCTCAAATTACTCTCAAAAATTGCATAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT171682 NZ_CP060948:c85203-85144 [Escherichia coli O157:H7 str. EC10]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin