Detailed information of TA system TA171679

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 45674..45943
• Replicon: plasmid pEC10-3
• Accession: NZ_CP060948
• Organism: Escherichia coli O157:H7 str. EC10

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: H9197_RS23790
• Protein ID: WP_001372321.1
• Coordinates: 45818..45943 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	45674..45739 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9197_RS23760	41384..41911	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
H9197_RS23765	41969..42202	+	234	WP_000006003.1	DUF905 family protein	-
H9197_RS23770	42263..44286	+	2024	Protein_54	ParB/RepB/Spo0J family partition protein	-
H9197_RS23775	44355..44789	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
H9197_RS23780	44786..45548	+	763	Protein_56	plasmid SOS inhibition protein A	-
-	45517..45741	+	225	NuclAT_0	-	-
-	45517..45741	+	225	NuclAT_0	-	-
-	45517..45741	+	225	NuclAT_0	-	-
-	45517..45741	+	225	NuclAT_0	-	-
-	45674..45739	-	66	-	-	Antitoxin
H9197_RS23785	45727..45876	+	150	Protein_57	plasmid maintenance protein Mok	-
H9197_RS23790	45818..45943	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
H9197_RS23795	46262..46558	-	297	Protein_59	hypothetical protein	-
H9197_RS23800	46858..47154	+	297	WP_001272251.1	hypothetical protein	-
H9197_RS23805	47265..48086	+	822	WP_001234445.1	DUF932 domain-containing protein	-
H9197_RS23810	48383..49030	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
H9197_RS23815	49307..49690	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
H9197_RS23820	49881..50567	+	687	WP_015059009.1	PAS domain-containing protein	-
H9197_RS23825	50662..50889	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	fosA3 / mph(A)	-	1..86240	86240

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T171679 WP_001372321.1 NZ_CP060948:45818-45943 [Escherichia coli O157:H7 str. EC10]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T171679 NZ_CP078017:c2175971-2175645 [Alcaligenes aquatilis]
ATGAGGCGTGGGGATCTAGTTACTGTTGCAATGCAAGGGGATTTTGGTAAACCTCGGCCTGCGTTAGTGATCCAGGCGGA
TTTATTTGCTGCTCATTCCTCGGTAACTGTGCTTCCAGTGACCAGCACAATTGTGAATGCGCCCTTATTGCGTATTAGCA
TTCAGCCAGATCTAGAAAATGGTCTACAAAAACCATCACAAATCATGATCGATAAGGCATTAACCGTTAAACGGGATAAG
ATTGGCCAAACCTTTGGATCTGTTAGTGCTGATACACTGGTAGAAATTGAACGGTGCCTTGCTGTTTTCTTGGGAATCGC
TAAGTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT171679 NZ_CP060948:c45739-45674 [Escherichia coli O157:H7 str. EC10]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin