Detailed information of TA system TA171673

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 42698..42962
• Replicon: plasmid pEC10-2
• Accession: NZ_CP060947
• Organism: Escherichia coli O157:H7 str. EC10

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: H9197_RS23265
• Protein ID: WP_001387489.1
• Coordinates: 42810..42962 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	42698..42758 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9197_RS23250 (38800)	38800..39870	-	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-
H9197_RS23255 (39889)	39889..41097	-	1209	WP_001383960.1	IncI1-type conjugal transfer protein TrbA	-
H9197_RS23260 (41404)	41404..42183	-	780	WP_275450201.1	protein FinQ	-
- (42698)	42698..42758	-	61	NuclAT_0	-	Antitoxin
- (42698)	42698..42758	-	61	NuclAT_0	-	Antitoxin
- (42698)	42698..42758	-	61	NuclAT_0	-	Antitoxin
- (42698)	42698..42758	-	61	NuclAT_0	-	Antitoxin
H9197_RS23265 (42810)	42810..42962	+	153	WP_001387489.1	Hok/Gef family protein	Toxin
H9197_RS23270 (43034)	43034..43285	-	252	WP_001291964.1	hypothetical protein	-
H9197_RS24530 (43786)	43786..43881	+	96	WP_000609148.1	DinQ-like type I toxin DqlB	-
H9197_RS23280 (43946)	43946..44122	-	177	WP_001054897.1	hypothetical protein	-
H9197_RS23285 (44514)	44514..44723	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
H9197_RS23290 (44795)	44795..45457	-	663	WP_000644796.1	plasmid IncI1-type surface exclusion protein ExcA	-
H9197_RS23295 (45528)	45528..47696	-	2169	WP_058649949.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..87111	87111

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T171673 WP_001387489.1 NZ_CP060947:42810-42962 [Escherichia coli O157:H7 str. EC10]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T171673 NZ_CP078015:c2803405-2803064 [Enterococcus faecalis]
ATGAATGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Nucleotide

• Length: 61 bp

>AT171673 NZ_CP060947:c42758-42698 [Escherichia coli O157:H7 str. EC10]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin