Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4074768..4075309 Replicon chromosome
Accession NZ_CP060946
Organism Escherichia coli O157:H7 str. EC10

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag H9197_RS19590 Protein ID WP_000615976.1
Coordinates 4074768..4075046 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag H9197_RS19595 Protein ID WP_000729704.1
Coordinates 4075049..4075309 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9197_RS19575 (4072350) 4072350..4072928 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
H9197_RS19580 (4073134) 4073134..4073901 + 768 WP_000333380.1 class II glutamine amidotransferase -
H9197_RS19585 (4073872) 4073872..4074612 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
H9197_RS19590 (4074768) 4074768..4075046 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
H9197_RS19595 (4075049) 4075049..4075309 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
H9197_RS19600 (4075495) 4075495..4076268 + 774 WP_000543899.1 C40 family peptidase -
H9197_RS19605 (4076325) 4076325..4076681 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
H9197_RS19610 (4076674) 4076674..4076952 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
H9197_RS19615 (4077057) 4077057..4079150 - 2094 WP_023155854.1 flagellar biosynthesis protein FlhA -
H9197_RS19620 (4079134) 4079134..4080273 - 1140 WP_023155855.1 flagellar type III secretion system protein FlhB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T171669 WP_000615976.1 NZ_CP060946:c4075046-4074768 [Escherichia coli O157:H7 str. EC10]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T171669 NZ_CP078015:396738-397013 [Enterococcus faecalis]
ATGCTTGAAATATTTTACACGAACCAATTCAAAAAAGACTTTAAGAAAGCAAAGAAACAAGGAAAGAATTTAGAGAAGTT
AAAAGAAGTATTAGTACTTTTACAAGAACAGCAAACATTGCCGCCAAAGTATAAAGACCACGCACTAACAGGAAATTATA
TAGGTACAAGAGAGTGCCATATTGAACCAGATTGGTTACTGATTTATAAAATTGACGGTGATAAATTGATCCTAACTTTA
GCTCGAATAGGCTCACATAGTGAATTATTTCGATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT171669 WP_000729704.1 NZ_CP060946:c4075309-4075049 [Escherichia coli O157:H7 str. EC10]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT171669 NZ_CP078015:396479-396745 [Enterococcus faecalis]
GTGGCTACTACAAAAAAGAAACCTATACATGTAAATGTTGATGAAAATTTAAAAGAAGAAGCAGAACAATTATTTGATGA
TTTAGGGTTAAATATGACAAGTGCAATTACGATTTTCTTAAAACAGTCTATTAATGAGCAAGCAATTCCTTTTATGATTA
ATAAGGGAAACAAAGAGACTCTACAAGCATTAAAAGACATTAAAGAAGGAAATGTTCATGGTGGATTTTCTTCCGTGGAG
GATTTAATGGAGGATTTAAATGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References