Detailed information of TA system TA171665

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3200102..3200704
• Replicon: chromosome
• Accession: NZ_CP060946
• Organism: Escherichia coli O157:H7 str. EC10

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: H9197_RS15450
• Protein ID: WP_000897305.1
• Coordinates: 3200102..3200413 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H9197_RS15455
• Protein ID: WP_000356397.1
• Coordinates: 3200414..3200704 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9197_RS15420 (3195132)	3195132..3195917	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
H9197_RS15425 (3196016)	3196016..3196615	+	600	WP_001295269.1	glucose-1-phosphatase	-
H9197_RS15430 (3196609)	3196609..3197481	+	873	WP_000920762.1	virulence factor BrkB family protein	-
H9197_RS15435 (3197478)	3197478..3197915	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
H9197_RS15440 (3197960)	3197960..3198901	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
H9197_RS15445 (3198965)	3198965..3199873	-	909	WP_001162704.1	alpha/beta hydrolase	-
H9197_RS15450 (3200102)	3200102..3200413	+	312	WP_000897305.1	hypothetical protein	Toxin
H9197_RS15455 (3200414)	3200414..3200704	+	291	WP_000356397.1	NadS family protein	Antitoxin
H9197_RS15460 (3201290)	3201290..3201508	+	219	WP_001295676.1	CopG family transcriptional regulator	-
H9197_RS15465 (3201727)	3201727..3201969	+	243	WP_001086388.1	protein YiiF	-
H9197_RS15470 (3202299)	3202299..3203228	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
H9197_RS15475 (3203225)	3203225..3203860	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
H9197_RS15480 (3203857)	3203857..3204759	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T171665 WP_000897305.1 NZ_CP060946:3200102-3200413 [Escherichia coli O157:H7 str. EC10]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T171665 NZ_CP078012:1500532-1500792 [Streptococcus equi subsp. zooepidemicus]
ATGTATCATATAGAGTATTCTAAAAAGGCTCAAAAGCAGATTAAGAAAGTAGATAAGCAAATTCAGAGGTTGCTATTTGC
TTGGATTGATAAGCATTTAGAGGGAACAGATAACCCACGCGCAAATGGTAAGGGTTTGGCAGGAAATCATGCTAATGAGT
GGCGTTATCGTATTGGTGATTATCGGCTTATTTGTGATATTCAAGATGATAAGATGGTTATTTTAGCGCTTGAGTTTGGG
CATTGTAAAGATATTTACTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT171665 WP_000356397.1 NZ_CP060946:3200414-3200704 [Escherichia coli O157:H7 str. EC10]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT171665 NZ_CP078012:1500309-1500530 [Streptococcus equi subsp. zooepidemicus]
ATGAGTACAGTTACTATCAGATTAAATCAAGAAGAAGAAATATTTTTCAAAAGTTATGCTCAACTCACAGGGCAAAGTTT
ATCAAGTCTATTTAAAAAAGCTCTTGAGCGGGATATTGAAGATGAGTATGATTTAAAACTCTATCATCAAGCCTATGCTG
AATATAAAGCAGACCCTGAGACAATTAGCCATGCGGATTTTAAGAAAGAGCTAGGCTTATAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7