Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2409063..2409862 Replicon chromosome
Accession NZ_CP060946
Organism Escherichia coli O157:H7 str. EC10

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag H9197_RS11605 Protein ID WP_000347267.1
Coordinates 2409398..2409862 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag H9197_RS11600 Protein ID WP_001307405.1
Coordinates 2409063..2409398 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9197_RS11585 (2404849) 2404849..2405619 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
H9197_RS11590 (2405635) 2405635..2406969 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
H9197_RS11595 (2407344) 2407344..2408915 + 1572 WP_001273741.1 galactarate dehydratase -
H9197_RS11600 (2409063) 2409063..2409398 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
H9197_RS11605 (2409398) 2409398..2409862 + 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
H9197_RS11610 (2409917) 2409917..2410726 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
H9197_RS11615 (2410975) 2410975..2412255 + 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
H9197_RS11620 (2412278) 2412278..2412751 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
H9197_RS11625 (2412762) 2412762..2413541 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
H9197_RS11630 (2413531) 2413531..2414409 + 879 WP_001298758.1 PTS N-acetylgalactosamine transporter subunit IID -
H9197_RS11635 (2414427) 2414427..2414861 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2398408..2409862 11454


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T171663 WP_000347267.1 NZ_CP060946:2409398-2409862 [Escherichia coli O157:H7 str. EC10]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T171663 NZ_CP078009:c5320767-5320468 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT171663 WP_001307405.1 NZ_CP060946:2409063-2409398 [Escherichia coli O157:H7 str. EC10]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT171663 NZ_CP078009:c5320471-5320181 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References