Detailed information of TA system TA171644

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 50110..50374
• Replicon: plasmid pEC11-3
• Accession: NZ_CP060940
• Organism: Escherichia coli strain EC11

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: H9198_RS24190
• Protein ID: WP_001387489.1
• Coordinates: 50110..50262 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	50314..50374 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9198_RS24150 (45202)	45202..45735	-	534	WP_001221666.1	lipocalin family protein	-
H9198_RS24155 (45829)	45829..46974	-	1146	WP_039023136.1	extended-spectrum class C beta-lactamase CMY-42	-
H9198_RS24165 (48179)	48179..48277	+	99	Protein_60	ethanolamine utilization protein EutE	-
H9198_RS24170 (48349)	48349..48558	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
H9198_RS24175 (48950)	48950..49126	+	177	WP_001054897.1	hypothetical protein	-
H9198_RS24870 (49191)	49191..49286	-	96	WP_000609148.1	DinQ-like type I toxin DqlB	-
H9198_RS24185 (49787)	49787..50038	+	252	WP_001291964.1	hypothetical protein	-
H9198_RS24190 (50110)	50110..50262	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
- (50314)	50314..50374	+	61	NuclAT_0	-	Antitoxin
- (50314)	50314..50374	+	61	NuclAT_0	-	Antitoxin
- (50314)	50314..50374	+	61	NuclAT_0	-	Antitoxin
- (50314)	50314..50374	+	61	NuclAT_0	-	Antitoxin
H9198_RS24195 (50583)	50583..50957	+	375	WP_223349261.1	hypothetical protein	-
H9198_RS24200 (51110)	51110..52267	+	1158	Protein_67	IS1380-like element ISEcp1 family transposase	-
H9198_RS24205 (52323)	52323..53020	+	698	WP_095033700.1	IS1-like element IS1B family transposase	-
H9198_RS24210 (53031)	53031..53339	-	309	WP_039023235.1	transcription termination/antitermination NusG family protein	-
H9198_RS24215 (53781)	53781..54068	-	288	WP_000074855.1	conjugal transfer protein TraA	-
H9198_RS24220 (54106)	54106..54264	-	159	Protein_71	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-14 / blaCMY-42	-	1..55175	55175
	-	inside	IScluster/Tn	blaCMY-42	-	40468..52598	12130

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T171644 WP_001387489.1 NZ_CP060940:c50262-50110 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T171644 NZ_CP078006:1503082-1503837 [Pseudomonas aeruginosa]
GTGAGCGAGATCTGGCGACAGTGCAAGGGTGAGCGGCATATCCGACCGCTCCAGGGGCGCCTGGTGCGGTTGGTGGAGAG
CCAGGAGCAGGTGGCGACCCTGCAATTGGTCGACACCCTGGAAGAACAGGCGTTGCTCGAGGAACTGCTCGAGTCGAGCA
AGCCGCCGGTGCCGGCGGATGCCGAGCCATTGCATTACCTGTTGAAGACCCCGTTCCGTTATCCGCCCTTGCGCTGGGGG
TCGCGGTTTGGCCGTCGCCACGAGCCAAGCCTGTTCTACGCCGCGCTGAAGCTGGAAACGGCCATGGCCGAAAGCGCGTA
TTACCGTTGCGTCCTGTGGAGTGGCATGGTGGTGCCGCCACCGAGCGGGAGGATTCTGTCCGAGCACGCTTCGTTCGAGG
CGGGCTGGAAGGTGGAGCGGGGAATCCGTCTGCAGGCGCCGCCTTTCTCCGATCACGAGGCTGCGCTGACCGACATCGCC
GACTACCGCGCTCCCCAGGAACTGGGAAGTGCCATGCGCAGCGCCGGGGTGCAGGCATTCGAATACCGTTCGGCGCGTTG
CCCGGAGCGCGGCTGCAATGTCGCCCTGTTCACGCCCGCGGCCTTCACCGAAAAGCGTCCTCGCAACCTGACGCCCTGGC
TGTGCGAAACCACGGCCGGTTACGTGGCCTTCAAGCCGGCGCATGTGCCGGGGTCGCCGAAAATCTTCTCCTGGGAGCTG
TTCCTGGTGGACGGAAAGTTGCCGCATCCGGCGTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT171644 NZ_CP060940:50314-50374 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin