Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4384638..4385179 Replicon chromosome
Accession NZ_CP060938
Organism Escherichia coli strain EC11

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag H9198_RS21210 Protein ID WP_000615983.1
Coordinates 4384901..4385179 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag H9198_RS21205 Protein ID WP_000729703.1
Coordinates 4384638..4384898 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9198_RS21185 (4380313) 4380313..4381098 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
H9198_RS21190 (4381070) 4381070..4382782 + 1713 Protein_4149 flagellar biosynthesis protein FlhA -
H9198_RS21195 (4383006) 4383006..4383503 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
H9198_RS21200 (4383679) 4383679..4384428 - 750 WP_000056849.1 C40 family peptidase -
H9198_RS21205 (4384638) 4384638..4384898 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
H9198_RS21210 (4384901) 4384901..4385179 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
H9198_RS21215 (4385335) 4385335..4386075 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
H9198_RS21220 (4386046) 4386046..4386813 - 768 WP_000333380.1 class II glutamine amidotransferase -
H9198_RS21225 (4387019) 4387019..4387597 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / rhs/PAAR / vgrG/tssI / hcp2/tssD2 / tssB / tssC / tssC 4378441..4405613 27172


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T171640 WP_000615983.1 NZ_CP060938:4384901-4385179 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T171640 NZ_CP078004:c5651145-5650846 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT171640 WP_000729703.1 NZ_CP060938:4384638-4384898 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT171640 NZ_CP078004:c5650849-5650559 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References