Detailed information of TA system TA171639

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4378441..4379135
• Replicon: chromosome
• Accession: NZ_CP060938
• Organism: Escherichia coli strain EC11

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: H9198_RS21170
• Protein ID: WP_001263489.1
• Coordinates: 4378441..4378839 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: H9198_RS21175
• Protein ID: WP_000554758.1
• Coordinates: 4378842..4379135 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (4374029)	4374029..4374109	-	81	NuclAT_12	-	-
- (4374029)	4374029..4374109	-	81	NuclAT_12	-	-
- (4374029)	4374029..4374109	-	81	NuclAT_12	-	-
- (4374029)	4374029..4374109	-	81	NuclAT_12	-	-
H9198_RS21145 (4374705)	4374705..4375163	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
H9198_RS21150 (4375424)	4375424..4376881	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
H9198_RS21155 (4376938)	4376938..4377459	-	522	Protein_4142	peptide chain release factor H	-
H9198_RS21160 (4377455)	4377455..4377661	-	207	Protein_4143	RtcB family protein	-
H9198_RS21165 (4377979)	4377979..4378431	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
H9198_RS21170 (4378441)	4378441..4378839	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
H9198_RS21175 (4378842)	4378842..4379135	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
H9198_RS21180 (4379187)	4379187..4380242	-	1056	WP_001226164.1	DNA polymerase IV	-
H9198_RS21185 (4380313)	4380313..4381098	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
H9198_RS21190 (4381070)	4381070..4382782	+	1713	Protein_4149	flagellar biosynthesis protein FlhA	-
H9198_RS21195 (4383006)	4383006..4383503	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA / rhs/PAAR / vgrG/tssI / hcp2/tssD2 / tssB / tssC / tssC	4378441..4405613	27172

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T171639 WP_001263489.1 NZ_CP060938:c4378839-4378441 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T171639 NZ_CP078004:c5650849-5650559 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT171639 WP_000554758.1 NZ_CP060938:c4379135-4378842 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT171639 NZ_CP078004:c5651145-5650846 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3