Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1307685..1308412 Replicon chromosome
Accession NZ_CP060938
Organism Escherichia coli strain EC11

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag H9198_RS06315 Protein ID WP_000550189.1
Coordinates 1307685..1307999 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag H9198_RS06320 Protein ID WP_000560266.1
Coordinates 1307996..1308412 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9198_RS06295 (1303843) 1303843..1304829 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
H9198_RS06300 (1304908) 1304908..1305600 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
H9198_RS06305 (1305677) 1305677..1306180 - 504 WP_001333820.1 M48 family metallopeptidase -
H9198_RS06310 (1306265) 1306265..1307401 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
H9198_RS06315 (1307685) 1307685..1307999 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
H9198_RS06320 (1307996) 1307996..1308412 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
H9198_RS06325 (1308457) 1308457..1310475 - 2019 WP_000121435.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
H9198_RS06330 (1310901) 1310901..1313252 - 2352 WP_000695487.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T171623 WP_000550189.1 NZ_CP060938:1307685-1307999 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T171623 NZ_CP078000:c5666-5391 [Pseudomonas aeruginosa]
ATGGAGTTGAAGTGGACGAGCAAAGGGCTCTCCGACGTGGCACGGCTCTACGAGTTCCTGGCTACGGTGAACAGGGAAGC
TGCCGCCCGCACGGTGCAGCAGCTGACGAGCGCGCCGACCGCCTTGTTGACCAACCCGCGGCTGGGCGAGCGCCTGGAAG
AGTTCGAACCGCGGGATGTTCGCCGCATCCTGATCGGCCACTATGAGATGCGCTATGAGATCCTGGATTCCTGCATCTAC
GTGCTTCGTCTGTGGCACACACGCGAAGACAGGTAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT171623 WP_000560266.1 NZ_CP060938:1307996-1308412 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT171623 NZ_CP078000:c5941-5666 [Pseudomonas aeruginosa]
ATGAGCACAGCAGCCAAACCAACGCGTACCGTGACTGCTCACGTCCCGGTAGATCTGGCCGAGCGCGTCGATGCAATCGC
CGAGCGCCTGGAGCGATCGAAGAACTGGATCGTCAGGCAGGCGCTGTCAGCCTGGATCGACCAGGAAGACGAGCGTAGCC
GTCTGACACGTGAAGCCTTGGATGATGTAGACGCAGGACGTGTCGTCGACCACCAGGCCGTCCAGGCCTGGGCCGAAAGC
CTGGGCACTGACTCTCCGCTGCCGGTACCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References