171622

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1263562..1264361 Replicon chromosome
Accession NZ_CP060938
Organism Escherichia coli strain EC11

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag H9198_RS06085 Protein ID WP_000347275.1
Coordinates 1263562..1264026 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag H9198_RS06090 Protein ID WP_001307405.1
Coordinates 1264026..1264361 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9198_RS06055 (1258563) 1258563..1258997 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
H9198_RS06060 (1259015) 1259015..1259893 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
H9198_RS06065 (1259883) 1259883..1260662 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
H9198_RS06070 (1260673) 1260673..1261146 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
H9198_RS06075 (1261169) 1261169..1262449 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
H9198_RS06080 (1262698) 1262698..1263507 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
H9198_RS06085 (1263562) 1263562..1264026 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
H9198_RS06090 (1264026) 1264026..1264361 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
H9198_RS06095 (1264510) 1264510..1266081 - 1572 WP_001273773.1 galactarate dehydratase -
H9198_RS06100 (1266456) 1266456..1267790 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
H9198_RS06105 (1267806) 1267806..1268576 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T171622 WP_000347275.1 NZ_CP060938:c1264026-1263562 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T171622 NZ_CP077999:c5922609-5922331 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTTGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT171622 WP_001307405.1 NZ_CP060938:c1264361-1264026 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT171622 NZ_CP077999:c5922319-5922014 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References