Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 832145..832944 Replicon chromosome
Accession NZ_CP060933
Organism Escherichia coli strain EC12

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag H9199_RS04105 Protein ID WP_000347266.1
Coordinates 832145..832609 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag H9199_RS04110 Protein ID WP_001307405.1
Coordinates 832609..832944 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9199_RS04075 (827146) 827146..827580 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
H9199_RS04080 (827598) 827598..828476 - 879 WP_001315856.1 PTS N-acetylgalactosamine transporter subunit IID -
H9199_RS04085 (828466) 828466..829245 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
H9199_RS04090 (829256) 829256..829729 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
H9199_RS04095 (829752) 829752..831032 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
H9199_RS04100 (831281) 831281..832090 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
H9199_RS04105 (832145) 832145..832609 - 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
H9199_RS04110 (832609) 832609..832944 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
H9199_RS04115 (833093) 833093..834664 - 1572 WP_001273741.1 galactarate dehydratase -
H9199_RS04120 (835039) 835039..836373 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
H9199_RS04125 (836389) 836389..837159 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 832145..843600 11455


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T171592 WP_000347266.1 NZ_CP060933:c832609-832145 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T171592 NZ_CP077988:1299793-1300107 [Pseudomonas aeruginosa]
ATGATCTTCATCGAAACGCCAGTCTTCACGAAGCGGATTCTCGCGCTGGTAGACGACGAGACCTATCGCAAGCTTCAGGA
AGACCTAACGCTCCACCCGGACGCCGGAGTGGTCATTGAAGGCACGGGTGGCGTGCGCAAGATTCGCATTGCTGCCAATG
GCCACGGGAAAAGAGGCGGCGCCCGGGTCATCTACTACCATTTCACATCCGCCTCTCAAATTGCATTCCTGCTGGCCTAT
GACAAAGCATCGCAGGAAGATCTGACTGCCGATCAGAAAAAGATGCTTCGGCAGATCGTTGAGAACTGGAGATAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT171592 WP_001307405.1 NZ_CP060933:c832944-832609 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT171592 NZ_CP077988:1300110-1300415 [Pseudomonas aeruginosa]
ATGGACAAAGAACTCTTCGCTGAACTGGTCGAAAGCGTGACCCAGATGGACGAGATCGTGCGTGGCGAACGTCAACCTTC
CCGCGAGTTCCACGTCGACGCGGTGAAGGTCAAGGAAATCCGCCGCGCTACTGGCCTGTCCCAGGCCAAATTCGCCCGAA
AGATCGATGTTGCTGTTGGAACCCTGCGCAATTGGGAGCAAGGGCGCCGCGATCCCGAGGGGCCTGCCCGCGCCCTGCTC
CGGGCCATTGACAATGATCCGGTTCATGTTCTGGCTGCCCTGAATGGTGGCCCGCGAGAAAGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References