Detailed information of TA system TA171581

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 81934..82188
• Replicon: plasmid pEC13-2
• Accession: NZ_CP060929
• Organism: Escherichia coli strain EC13

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: H9200_RS23375
• Protein ID: WP_001312851.1
• Coordinates: 81934..82083 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	82127..82188 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9200_RS23330 (77496)	77496..77897	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
H9200_RS23335 (77830)	77830..78087	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
H9200_RS23340 (78180)	78180..78488	-	309	WP_226116723.1	CPBP family intramembrane metalloprotease	-
H9200_RS23345 (78430)	78430..78822	-	393	WP_000616804.1	histidine phosphatase family protein	-
H9200_RS23350 (79762)	79762..80619	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
H9200_RS23355 (80612)	80612..81094	-	483	WP_001273588.1	hypothetical protein	-
H9200_RS23360 (81087)	81087..81134	-	48	WP_229471593.1	hypothetical protein	-
H9200_RS23365 (81125)	81125..81376	+	252	WP_223195197.1	replication protein RepA	-
H9200_RS23370 (81393)	81393..81650	-	258	WP_000083845.1	replication regulatory protein RepA	-
H9200_RS23375 (81934)	81934..82083	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (82127)	82127..82188	+	62	NuclAT_1	-	Antitoxin
- (82127)	82127..82188	+	62	NuclAT_1	-	Antitoxin
- (82127)	82127..82188	+	62	NuclAT_1	-	Antitoxin
- (82127)	82127..82188	+	62	NuclAT_1	-	Antitoxin
H9200_RS23380 (82444)	82444..82518	-	75	Protein_90	endonuclease	-
H9200_RS23385 (82764)	82764..82976	-	213	WP_001299730.1	ANR family transcriptional regulator	-
H9200_RS23390 (83112)	83112..83672	-	561	WP_000139315.1	fertility inhibition protein FinO	-
H9200_RS23395 (83775)	83775..84635	-	861	WP_000704514.1	alpha/beta hydrolase	-
H9200_RS23400 (84694)	84694..85440	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-14 / fosA3 / aac(3)-IVa / sul2 / floR / dfrA17 / oqxB / oqxA / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..145694	145694

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T171581 WP_001312851.1 NZ_CP060929:c82083-81934 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T171581 NZ_CP077981:c4743110-4742745 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGAGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCATG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGACCCCAGACGCTCCGCG
ATACTGCTGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCTATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT171581 NZ_CP060929:82127-82188 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin