Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1186243..1186745 Replicon chromosome
Accession NZ_CP060928
Organism Escherichia coli strain EC13

Toxin (Protein)


Gene name yoeB Uniprot ID A0A8E0FJZ4
Locus tag H9200_RS06065 Protein ID WP_000767833.1
Coordinates 1186243..1186497 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag H9200_RS06070 Protein ID WP_001259255.1
Coordinates 1186494..1186745 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9200_RS06035 (1181258) 1181258..1181485 - 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
H9200_RS06040 (1181499) 1181499..1182557 - 1059 WP_000492324.1 thiosulfate utilization transporter TsuA/YeeE -
H9200_RS06045 (1182736) 1182736..1184094 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
H9200_RS06050 (1184084) 1184084..1184146 - 63 WP_010723108.1 membrane protein YoeI -
H9200_RS06055 (1184361) 1184361..1185290 - 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
H9200_RS06060 (1185336) 1185336..1186160 - 825 WP_000754758.1 SDR family oxidoreductase -
H9200_RS06065 (1186243) 1186243..1186497 - 255 WP_000767833.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
H9200_RS06070 (1186494) 1186494..1186745 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
H9200_RS06075 (1187028) 1187028..1187078 + 51 WP_001364200.1 his operon leader peptide -
H9200_RS06080 (1187224) 1187224..1188123 + 900 WP_000131756.1 ATP phosphoribosyltransferase -
H9200_RS06085 (1188129) 1188129..1189427 + 1299 WP_049290931.1 histidinol dehydrogenase -
H9200_RS06090 (1189430) 1189430..1190500 + 1071 WP_000108939.1 histidinol-phosphate transaminase -
H9200_RS06095 (1190500) 1190500..1191567 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10173.54 Da        Isoelectric Point: 8.0353

>T171570 WP_000767833.1 NZ_CP060928:c1186497-1186243 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPESLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T171570 NZ_CP077977:c145592-145311 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGAAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT171570 WP_001259255.1 NZ_CP060928:c1186745-1186494 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT171570 NZ_CP077977:c145816-145589 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACATCGAGATCGAAAAGCGCTGGGGGCTGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References