Detailed information of TA system TA171570

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1186243..1186745
• Replicon: chromosome
• Accession: NZ_CP060928
• Organism: Escherichia coli strain EC13

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A8E0FJZ4
• Locus tag: H9200_RS06065
• Protein ID: WP_000767833.1
• Coordinates: 1186243..1186497 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: H9200_RS06070
• Protein ID: WP_001259255.1
• Coordinates: 1186494..1186745 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9200_RS06035 (1181258)	1181258..1181485	-	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-
H9200_RS06040 (1181499)	1181499..1182557	-	1059	WP_000492324.1	thiosulfate utilization transporter TsuA/YeeE	-
H9200_RS06045 (1182736)	1182736..1184094	-	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
H9200_RS06050 (1184084)	1184084..1184146	-	63	WP_010723108.1	membrane protein YoeI	-
H9200_RS06055 (1184361)	1184361..1185290	-	930	WP_000803351.1	LysR substrate-binding domain-containing protein	-
H9200_RS06060 (1185336)	1185336..1186160	-	825	WP_000754758.1	SDR family oxidoreductase	-
H9200_RS06065 (1186243)	1186243..1186497	-	255	WP_000767833.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
H9200_RS06070 (1186494)	1186494..1186745	-	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
H9200_RS06075 (1187028)	1187028..1187078	+	51	WP_001364200.1	his operon leader peptide	-
H9200_RS06080 (1187224)	1187224..1188123	+	900	WP_000131756.1	ATP phosphoribosyltransferase	-
H9200_RS06085 (1188129)	1188129..1189427	+	1299	WP_049290931.1	histidinol dehydrogenase	-
H9200_RS06090 (1189430)	1189430..1190500	+	1071	WP_000108939.1	histidinol-phosphate transaminase	-
H9200_RS06095 (1190500)	1190500..1191567	+	1068	WP_000080093.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10173.54 Da
• Isoelectric Point: 8.0353

>T171570 WP_000767833.1 NZ_CP060928:c1186497-1186243 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPESLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T171570 NZ_CP077977:c145592-145311 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGAAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT171570 WP_001259255.1 NZ_CP060928:c1186745-1186494 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT171570 NZ_CP077977:c145816-145589 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACATCGAGATCGAAAAGCGCTGGGGGCTGCAATGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LNR0