Detailed information of TA system TA171547

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4627904..4628506
• Replicon: chromosome
• Accession: NZ_CP060924
• Organism: Escherichia coli strain EC14

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: H9201_RS22355
• Protein ID: WP_000897305.1
• Coordinates: 4628195..4628506 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H9201_RS22350
• Protein ID: WP_000356397.1
• Coordinates: 4627904..4628194 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9201_RS22325 (4623849)	4623849..4624751	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
H9201_RS22330 (4624748)	4624748..4625383	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
H9201_RS22335 (4625380)	4625380..4626309	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
H9201_RS22340 (4626639)	4626639..4626881	-	243	WP_001087409.1	protein YiiF	-
H9201_RS22345 (4627100)	4627100..4627318	-	219	WP_001295676.1	CopG family transcriptional regulator	-
H9201_RS22350 (4627904)	4627904..4628194	-	291	WP_000356397.1	NadS family protein	Antitoxin
H9201_RS22355 (4628195)	4628195..4628506	-	312	WP_000897305.1	hypothetical protein	Toxin
H9201_RS22360 (4628735)	4628735..4629643	+	909	WP_001162704.1	alpha/beta hydrolase	-
H9201_RS22365 (4629707)	4629707..4630648	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
H9201_RS22370 (4630693)	4630693..4631130	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
H9201_RS22375 (4631127)	4631127..4631999	-	873	WP_000920762.1	virulence factor BrkB family protein	-
H9201_RS22380 (4631993)	4631993..4632592	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T171547 WP_000897305.1 NZ_CP060924:c4628506-4628195 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T171547 NZ_CP077969:c1842020-1841917 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT171547 WP_000356397.1 NZ_CP060924:c4628194-4627904 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT171547 NZ_CP077969:1841879-1842150 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7