Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3765281..3765822 Replicon chromosome
Accession NZ_CP060924
Organism Escherichia coli strain EC14

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag H9201_RS18290 Protein ID WP_000615982.1
Coordinates 3765544..3765822 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag H9201_RS18285 Protein ID WP_000729704.1
Coordinates 3765281..3765541 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9201_RS18260 (3760317) 3760317..3761456 + 1140 WP_000785662.1 flagellar type III secretion system protein FlhB -
H9201_RS18265 (3761440) 3761440..3763533 + 2094 WP_001140806.1 flagellar biosynthesis protein FlhA -
H9201_RS18270 (3763638) 3763638..3763916 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
H9201_RS18275 (3763909) 3763909..3764265 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
H9201_RS18280 (3764322) 3764322..3765095 - 774 WP_000543899.1 C40 family peptidase -
H9201_RS18285 (3765281) 3765281..3765541 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
H9201_RS18290 (3765544) 3765544..3765822 + 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
H9201_RS18295 (3765978) 3765978..3766718 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
H9201_RS18300 (3766689) 3766689..3767456 - 768 WP_000333380.1 class II glutamine amidotransferase -
H9201_RS18305 (3767662) 3767662..3768240 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T171542 WP_000615982.1 NZ_CP060924:3765544-3765822 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T171542 NZ_CP077969:c1139867-1139760 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT171542 WP_000729704.1 NZ_CP060924:3765281-3765541 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT171542 NZ_CP077969:1139914-1139980 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References