Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 630009..630808 Replicon chromosome
Accession NZ_CP060924
Organism Escherichia coli strain EC14

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag H9201_RS03065 Protein ID WP_000347266.1
Coordinates 630009..630473 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag H9201_RS03070 Protein ID WP_001307405.1
Coordinates 630473..630808 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9201_RS03035 (625010) 625010..625444 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
H9201_RS03040 (625462) 625462..626340 - 879 WP_001315856.1 PTS N-acetylgalactosamine transporter subunit IID -
H9201_RS03045 (626330) 626330..627109 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
H9201_RS03050 (627120) 627120..627593 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
H9201_RS03055 (627616) 627616..628896 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
H9201_RS03060 (629145) 629145..629954 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
H9201_RS03065 (630009) 630009..630473 - 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
H9201_RS03070 (630473) 630473..630808 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
H9201_RS03075 (630957) 630957..632528 - 1572 WP_001273741.1 galactarate dehydratase -
H9201_RS03080 (632903) 632903..634237 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
H9201_RS03085 (634253) 634253..635023 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T171524 WP_000347266.1 NZ_CP060924:c630473-630009 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T171524 NZ_CP077960:955234-955605 [Mammaliicoccus sciuri]
ATGAGAAGAGGAGATGTATATCTCGCAGATCTTTCACCAGTTACAGGTTCTGAACAAGGGGGGACAAGACCAGTCGTTAT
TATCCAGAATGATACAGGTAATCGATACAGCCCGACTGTGATTGTTGCAGCAATTACTGGTAAGATAAATAAAGCCAAAA
TTCCCACTCATGTAGAAATTGAGGCAGCGAAATATAAATTAGACAGAGATTCCGTAATTTTGCTTGAACAAATCAGGACA
ATTGATAAAAAACGCTTAAAAGAAAAATTAACATATTTATCTGATGCAAAGATGAAAGAGGTTGATATCGCTATTGCCAT
CAGCCTCAATCTAACTTTGCAACAAAAATTTGATACATTAGGTAATACATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT171524 WP_001307405.1 NZ_CP060924:c630808-630473 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT171524 NZ_CP077960:955066-955233 [Mammaliicoccus sciuri]
ATGACATCTTTAACGCAAAATCGTACACAAAGTTTAGAACAAACACTGAAAGAAGGCTATCGTTCAATGGCCGATTTAAA
TCTCTCCCTTGCATCTGAAGCATACTATTGTGAATGTGAAGCTTGTGATTCAAATGAATCGCATATAGCCTCTCAGCAAG
GTGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References