Detailed information of TA system TA171463

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 36775..37377
• Replicon: chromosome
• Accession: NZ_CP060914
• Organism: Escherichia coli strain EC18

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: H9203_RS00220
• Protein ID: WP_000897305.1
• Coordinates: 37066..37377 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H9203_RS00215
• Protein ID: WP_000356397.1
• Coordinates: 36775..37065 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9203_RS00190 (32720)	32720..33622	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
H9203_RS00195 (33619)	33619..34254	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
H9203_RS00200 (34251)	34251..35180	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
H9203_RS00205 (35510)	35510..35752	-	243	WP_001087409.1	protein YiiF	-
H9203_RS00210 (35971)	35971..36189	-	219	WP_001295676.1	CopG family transcriptional regulator	-
H9203_RS00215 (36775)	36775..37065	-	291	WP_000356397.1	NadS family protein	Antitoxin
H9203_RS00220 (37066)	37066..37377	-	312	WP_000897305.1	hypothetical protein	Toxin
H9203_RS00225 (37606)	37606..38514	+	909	WP_001162704.1	alpha/beta hydrolase	-
H9203_RS00230 (38578)	38578..39519	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
H9203_RS00235 (39564)	39564..40001	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
H9203_RS00240 (39998)	39998..40870	-	873	WP_000920762.1	virulence factor BrkB family protein	-
H9203_RS00245 (40864)	40864..41463	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	35510..45030	9520

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T171463 WP_000897305.1 NZ_CP060914:c37377-37066 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T171463 NZ_CP077925:c988854-988666 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT171463 WP_000356397.1 NZ_CP060914:c37065-36775 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT171463 NZ_CP077925:c989143-988883 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATAGTACCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7