Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 663749..664548 Replicon chromosome
Accession NZ_CP060902
Organism Escherichia coli strain EC20

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag H9205_RS03220 Protein ID WP_000347251.1
Coordinates 663749..664213 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag H9205_RS03225 Protein ID WP_001307405.1
Coordinates 664213..664548 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9205_RS03190 (658750) 658750..659184 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
H9205_RS03195 (659202) 659202..660080 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
H9205_RS03200 (660070) 660070..660849 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
H9205_RS03205 (660860) 660860..661333 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
H9205_RS03210 (661356) 661356..662636 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
H9205_RS03215 (662885) 662885..663694 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
H9205_RS03220 (663749) 663749..664213 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
H9205_RS03225 (664213) 664213..664548 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
H9205_RS03230 (664697) 664697..666268 - 1572 WP_001273761.1 galactarate dehydratase -
H9205_RS03235 (666643) 666643..667977 + 1335 WP_000599652.1 galactarate/glucarate/glycerate transporter GarP -
H9205_RS03240 (667993) 667993..668763 + 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T171403 WP_000347251.1 NZ_CP060902:c664213-663749 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T171403 NZ_CP077916:c708982-708875 [Staphylococcus aureus]
ATGTTCAATTTATTAATTAACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT171403 WP_001307405.1 NZ_CP060902:c664548-664213 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT171403 NZ_CP077916:708798-708858 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References