Detailed information of TA system TA171398

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 140019..140445
• Replicon: plasmid pEC21-2
• Accession: NZ_CP060900
• Organism: Escherichia coli strain EC21

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: H9206_RS23605
• Protein ID: WP_001372321.1
• Coordinates: 140019..140144 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	140221..140445 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9206_RS23560 (135065)	135065..135292	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
H9206_RS23565 (135386)	135386..136072	-	687	WP_000332484.1	PAS domain-containing protein	-
H9206_RS23570 (136263)	136263..136646	-	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
H9206_RS23575 (136923)	136923..137570	+	648	WP_000614936.1	transglycosylase SLT domain-containing protein	-
H9206_RS23580 (137867)	137867..138688	-	822	WP_001234469.1	DUF932 domain-containing protein	-
H9206_RS23585 (138810)	138810..139097	-	288	WP_000107535.1	hypothetical protein	-
H9206_RS23590 (139122)	139122..139328	-	207	WP_000547939.1	hypothetical protein	-
H9206_RS23595 (139398)	139398..139571	+	174	Protein_151	hypothetical protein	-
H9206_RS23600 (139569)	139569..139799	-	231	WP_001426396.1	hypothetical protein	-
H9206_RS23605 (140019)	140019..140144	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
H9206_RS23610 (140086)	140086..140235	-	150	Protein_154	plasmid maintenance protein Mok	-
- (140221)	140221..140445	-	225	NuclAT_0	-	Antitoxin
- (140221)	140221..140445	-	225	NuclAT_0	-	Antitoxin
- (140221)	140221..140445	-	225	NuclAT_0	-	Antitoxin
- (140221)	140221..140445	-	225	NuclAT_0	-	Antitoxin
H9206_RS23615 (140257)	140257..140445	+	189	WP_001299721.1	hypothetical protein	-
H9206_RS23620 (140414)	140414..141176	-	763	Protein_156	plasmid SOS inhibition protein A	-
H9206_RS23625 (141173)	141173..141607	-	435	WP_000845895.1	conjugation system SOS inhibitor PsiB	-
H9206_RS23630 (141662)	141662..143620	-	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
H9206_RS23635 (143679)	143679..143912	-	234	WP_000006004.1	DUF905 domain-containing protein	-
H9206_RS23640 (143968)	143968..144495	-	528	WP_000290793.1	single-stranded DNA-binding protein	-
H9206_RS23645 (144968)	144968..145228	-	261	WP_072132736.1	hypothetical protein	-
H9206_RS23650 (145138)	145138..145380	-	243	WP_001610271.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T171398 WP_001372321.1 NZ_CP060900:c140144-140019 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T171398 NZ_CP077916:c192665-192513 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGACTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAAAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT171398 NZ_CP060900:c140445-140221 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin