Detailed information of TA system TA171397

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4518247..4518849
• Replicon: chromosome
• Accession: NZ_CP060899
• Organism: Escherichia coli strain EC21

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: H9206_RS21860
• Protein ID: WP_000897305.1
• Coordinates: 4518538..4518849 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H9206_RS21855
• Protein ID: WP_000356397.1
• Coordinates: 4518247..4518537 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9206_RS21830 (4514192)	4514192..4515094	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
H9206_RS21835 (4515091)	4515091..4515726	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
H9206_RS21840 (4515723)	4515723..4516652	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
H9206_RS21845 (4516982)	4516982..4517224	-	243	WP_001087409.1	protein YiiF	-
H9206_RS21850 (4517443)	4517443..4517661	-	219	WP_001295676.1	CopG family transcriptional regulator	-
H9206_RS21855 (4518247)	4518247..4518537	-	291	WP_000356397.1	NadS family protein	Antitoxin
H9206_RS21860 (4518538)	4518538..4518849	-	312	WP_000897305.1	hypothetical protein	Toxin
H9206_RS21865 (4519078)	4519078..4519986	+	909	WP_001162704.1	alpha/beta hydrolase	-
H9206_RS21870 (4520050)	4520050..4520991	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
H9206_RS21875 (4521036)	4521036..4521473	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
H9206_RS21880 (4521470)	4521470..4522342	-	873	WP_000920762.1	virulence factor BrkB family protein	-
H9206_RS21885 (4522336)	4522336..4522935	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T171397 WP_000897305.1 NZ_CP060899:c4518849-4518538 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T171397 NZ_CP077915:2686029-2686181 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT171397 WP_000356397.1 NZ_CP060899:c4518537-4518247 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT171397 NZ_CP077915:2685405-2686004 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7