Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3659349..3659890 Replicon chromosome
Accession NZ_CP060899
Organism Escherichia coli strain EC21

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag H9206_RS17800 Protein ID WP_000615982.1
Coordinates 3659612..3659890 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag H9206_RS17795 Protein ID WP_000729704.1
Coordinates 3659349..3659609 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9206_RS17770 (3654385) 3654385..3655524 + 1140 WP_000785662.1 flagellar type III secretion system protein FlhB -
H9206_RS17775 (3655508) 3655508..3657601 + 2094 WP_001140806.1 flagellar biosynthesis protein FlhA -
H9206_RS17780 (3657706) 3657706..3657984 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
H9206_RS17785 (3657977) 3657977..3658333 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
H9206_RS17790 (3658390) 3658390..3659163 - 774 WP_000543899.1 C40 family peptidase -
H9206_RS17795 (3659349) 3659349..3659609 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
H9206_RS17800 (3659612) 3659612..3659890 + 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
H9206_RS17805 (3660046) 3660046..3660786 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
H9206_RS17810 (3660757) 3660757..3661524 - 768 WP_000333380.1 class II glutamine amidotransferase -
H9206_RS17815 (3661730) 3661730..3662308 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T171392 WP_000615982.1 NZ_CP060899:3659612-3659890 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T171392 NZ_CP077915:1039985-1040173 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT171392 WP_000729704.1 NZ_CP060899:3659349-3659609 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT171392 NZ_CP077915:1039696-1039956 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References