Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3791167..3791708 Replicon chromosome
Accession NZ_CP060892
Organism Escherichia coli strain EC22

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag H9207_RS18380 Protein ID WP_000615983.1
Coordinates 3791430..3791708 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag H9207_RS18375 Protein ID WP_000729703.1
Coordinates 3791167..3791427 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9207_RS18355 (3786842) 3786842..3787627 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
H9207_RS18360 (3787599) 3787599..3789311 + 1713 Protein_3618 flagellar biosynthesis protein FlhA -
H9207_RS18365 (3789535) 3789535..3790032 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
H9207_RS18370 (3790208) 3790208..3790957 - 750 WP_000056849.1 C40 family peptidase -
H9207_RS18375 (3791167) 3791167..3791427 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
H9207_RS18380 (3791430) 3791430..3791708 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
H9207_RS18385 (3791864) 3791864..3792604 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
H9207_RS18390 (3792575) 3792575..3793342 - 768 WP_000333380.1 class II glutamine amidotransferase -
H9207_RS18395 (3793548) 3793548..3794126 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T171366 WP_000615983.1 NZ_CP060892:3791430-3791708 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T171366 NZ_CP077899:c954004-953816 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGTTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGTAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT171366 WP_000729703.1 NZ_CP060892:3791167-3791427 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT171366 NZ_CP077899:c954293-954033 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATAGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References