Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3784970..3785664 Replicon chromosome
Accession NZ_CP060892
Organism Escherichia coli strain EC22

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag H9207_RS18340 Protein ID WP_001263489.1
Coordinates 3784970..3785368 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag H9207_RS18345 Protein ID WP_000554758.1
Coordinates 3785371..3785664 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3780558) 3780558..3780638 - 81 NuclAT_11 - -
- (3780558) 3780558..3780638 - 81 NuclAT_11 - -
- (3780558) 3780558..3780638 - 81 NuclAT_11 - -
- (3780558) 3780558..3780638 - 81 NuclAT_11 - -
H9207_RS18315 (3781234) 3781234..3781692 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
H9207_RS18320 (3781953) 3781953..3783410 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
H9207_RS18325 (3783467) 3783467..3783988 - 522 Protein_3611 peptide chain release factor H -
H9207_RS18330 (3783984) 3783984..3784190 - 207 Protein_3612 RtcB family protein -
H9207_RS18335 (3784508) 3784508..3784960 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
H9207_RS18340 (3784970) 3784970..3785368 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
H9207_RS18345 (3785371) 3785371..3785664 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
H9207_RS18350 (3785716) 3785716..3786771 - 1056 WP_001226164.1 DNA polymerase IV -
H9207_RS18355 (3786842) 3786842..3787627 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
H9207_RS18360 (3787599) 3787599..3789311 + 1713 Protein_3618 flagellar biosynthesis protein FlhA -
H9207_RS18365 (3789535) 3789535..3790032 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T171365 WP_001263489.1 NZ_CP060892:c3785368-3784970 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T171365 NZ_CP077898:c2409199-2409092 [Staphylococcus aureus]
ATGTTCAATTTATTAATTGACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT171365 WP_000554758.1 NZ_CP060892:c3785664-3785371 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT171365 NZ_CP077898:2409015-2409075 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References