Detailed information of TA system TA171348

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 722713..723406
• Replicon: chromosome
• Accession: NZ_CP060892
• Organism: Escherichia coli strain EC22

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: H9207_RS03530
• Protein ID: WP_000415584.1
• Coordinates: 722713..723009 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: H9207_RS03535
• Protein ID: WP_000650107.1
• Coordinates: 723011..723406 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9207_RS03495 (717801)	717801..718115	-	315	WP_000958598.1	putative quinol monooxygenase	-
H9207_RS03500 (718146)	718146..718727	-	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
H9207_RS03505 (719046)	719046..719378	+	333	WP_000917685.1	DUF2645 family protein	-
H9207_RS03510 (719424)	719424..720773	-	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
H9207_RS03515 (720770)	720770..721429	-	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	-
H9207_RS03520 (721581)	721581..721973	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
H9207_RS03525 (722026)	722026..722508	+	483	WP_000183505.1	GyrI-like domain-containing protein	-
H9207_RS03530 (722713)	722713..723009	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
H9207_RS03535 (723011)	723011..723406	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
H9207_RS03540 (723539)	723539..725146	+	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
H9207_RS03545 (725284)	725284..727542	+	2259	WP_032329482.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T171348 WP_000415584.1 NZ_CP060892:722713-723009 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T171348 NZ_CP077894:1383960-1384112 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGAGAGTCAGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT171348 WP_000650107.1 NZ_CP060892:723011-723406 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT171348 NZ_CP077894:1383336-1383935 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTACTAGATGAATTAAAGAAAAATGTAG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
CAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACTGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65