Detailed information of TA system TA171321

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1119246..1119973
• Replicon: chromosome
• Accession: NZ_CP060885
• Organism: Escherichia coli strain EC24

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: H9208_RS05430
• Protein ID: WP_000547564.1
• Coordinates: 1119246..1119557 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H9208_RS05435
• Protein ID: WP_000126294.1
• Coordinates: 1119554..1119973 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9208_RS05400 (1114388)	1114388..1116097	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
H9208_RS05405 (1116107)	1116107..1116649	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
H9208_RS05410 (1116649)	1116649..1117416	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
H9208_RS05415 (1117413)	1117413..1117823	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
H9208_RS05420 (1117816)	1117816..1118286	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
H9208_RS05425 (1118311)	1118311..1119072	+	762	WP_001026446.1	hypothetical protein	-
H9208_RS05430 (1119246)	1119246..1119557	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
H9208_RS05435 (1119554)	1119554..1119973	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
H9208_RS05440 (1120087)	1120087..1121511	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
H9208_RS05445 (1121520)	1121520..1122977	-	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
H9208_RS05450 (1123237)	1123237..1124247	+	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
H9208_RS05455 (1124396)	1124396..1124923	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T171321 WP_000547564.1 NZ_CP060885:1119246-1119557 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T171321 NZ_CP077888:c328408-328199 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT171321 WP_000126294.1 NZ_CP060885:1119554-1119973 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT171321 NZ_CP077888:c328713-328420 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5