Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3779222..3779763 Replicon chromosome
Accession NZ_CP060880
Organism Escherichia coli strain EC26

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag H9209_RS18470 Protein ID WP_000615983.1
Coordinates 3779485..3779763 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag H9209_RS18465 Protein ID WP_000729703.1
Coordinates 3779222..3779482 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9209_RS18445 (3774897) 3774897..3775682 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
H9209_RS18450 (3775654) 3775654..3777366 + 1713 Protein_3629 flagellar biosynthesis protein FlhA -
H9209_RS18455 (3777590) 3777590..3778087 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
H9209_RS18460 (3778263) 3778263..3779012 - 750 WP_000056849.1 C40 family peptidase -
H9209_RS18465 (3779222) 3779222..3779482 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
H9209_RS18470 (3779485) 3779485..3779763 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
H9209_RS18475 (3779919) 3779919..3780659 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
H9209_RS18480 (3780630) 3780630..3781397 - 768 WP_000333380.1 class II glutamine amidotransferase -
H9209_RS18485 (3781603) 3781603..3782181 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T171308 WP_000615983.1 NZ_CP060880:3779485-3779763 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T171308 NZ_CP077885:632025-632201 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT171308 WP_000729703.1 NZ_CP060880:3779222-3779482 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT171308 NZ_CP077885:c632363-632189 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAGTGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References