171307

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3773025..3773719 Replicon chromosome
Accession NZ_CP060880
Organism Escherichia coli strain EC26

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag H9209_RS18430 Protein ID WP_001263489.1
Coordinates 3773025..3773423 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag H9209_RS18435 Protein ID WP_000554758.1
Coordinates 3773426..3773719 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3768613) 3768613..3768693 - 81 NuclAT_11 - -
- (3768613) 3768613..3768693 - 81 NuclAT_11 - -
- (3768613) 3768613..3768693 - 81 NuclAT_11 - -
- (3768613) 3768613..3768693 - 81 NuclAT_11 - -
H9209_RS18405 (3769289) 3769289..3769747 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
H9209_RS18410 (3770008) 3770008..3771465 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
H9209_RS18415 (3771522) 3771522..3772043 - 522 Protein_3622 peptide chain release factor H -
H9209_RS18420 (3772039) 3772039..3772245 - 207 Protein_3623 RtcB family protein -
H9209_RS18425 (3772563) 3772563..3773015 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
H9209_RS18430 (3773025) 3773025..3773423 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
H9209_RS18435 (3773426) 3773426..3773719 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
H9209_RS18440 (3773771) 3773771..3774826 - 1056 WP_001226164.1 DNA polymerase IV -
H9209_RS18445 (3774897) 3774897..3775682 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
H9209_RS18450 (3775654) 3775654..3777366 + 1713 Protein_3629 flagellar biosynthesis protein FlhA -
H9209_RS18455 (3777590) 3777590..3778087 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T171307 WP_001263489.1 NZ_CP060880:c3773423-3773025 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T171307 NZ_CP077885:c573971-573783 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTAGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT171307 WP_000554758.1 NZ_CP060880:c3773719-3773426 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT171307 NZ_CP077885:c574260-574000 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References