Detailed information of TA system TA171277

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 67871..68125
• Replicon: plasmid pEC27-2
• Accession: NZ_CP060874
• Organism: Escherichia coli strain EC27

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: H9210_RS23655
• Protein ID: WP_001312851.1
• Coordinates: 67871..68020 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	68064..68125 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9210_RS23625 (63118)	63118..64029	-	912	WP_000440183.1	carbamate kinase	-
H9210_RS23630 (64040)	64040..65260	-	1221	WP_000410951.1	arginine deiminase	-
H9210_RS23635 (65967)	65967..66581	+	615	Protein_73	VENN motif pre-toxin domain-containing protein	-
H9210_RS23640 (66581)	66581..67027	-	447	Protein_74	plasmid replication initiator RepA	-
H9210_RS23645 (67020)	67020..67094	-	75	WP_032336874.1	RepA leader peptide Tap	-
H9210_RS23650 (67330)	67330..67587	-	258	WP_000083833.1	replication regulatory protein RepA	-
H9210_RS23655 (67871)	67871..68020	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (68064)	68064..68125	+	62	NuclAT_0	-	Antitoxin
- (68064)	68064..68125	+	62	NuclAT_0	-	Antitoxin
- (68064)	68064..68125	+	62	NuclAT_0	-	Antitoxin
- (68064)	68064..68125	+	62	NuclAT_0	-	Antitoxin
H9210_RS23660 (68264)	68264..68446	-	183	WP_000968309.1	hypothetical protein	-
H9210_RS23665 (68547)	68547..69163	+	617	Protein_79	IS1-like element IS1A family transposase	-
H9210_RS23670 (69201)	69201..70772	-	1572	WP_023149734.1	IS66-like element ISCro1 family transposase	-
H9210_RS23675 (70792)	70792..71139	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
H9210_RS23680 (71139)	71139..71816	-	678	WP_001339397.1	IS66-like element accessory protein TnpA	-
H9210_RS23685 (71871)	71871..71960	+	90	Protein_83	IS1 family transposase	-
H9210_RS23690 (72261)	72261..72473	-	213	WP_005012601.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / tet(A) / sitABCD	senB / iutA / iucD / iucC / iucB / iucA	1..111544	111544

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T171277 WP_001312851.1 NZ_CP060874:c68020-67871 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T171277 NZ_CP077872:2106-2468 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Nucleotide

• Length: 62 bp

>AT171277 NZ_CP060874:68064-68125 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin