Detailed information of TA system TA171262

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1119259..1119986
• Replicon: chromosome
• Accession: NZ_CP060873
• Organism: Escherichia coli strain EC27

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: H9210_RS05425
• Protein ID: WP_000547564.1
• Coordinates: 1119259..1119570 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H9210_RS05430
• Protein ID: WP_000126294.1
• Coordinates: 1119567..1119986 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9210_RS05395 (1114401)	1114401..1116110	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
H9210_RS05400 (1116120)	1116120..1116662	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
H9210_RS05405 (1116662)	1116662..1117429	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
H9210_RS05410 (1117426)	1117426..1117836	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
H9210_RS05415 (1117829)	1117829..1118299	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
H9210_RS05420 (1118324)	1118324..1119085	+	762	WP_001026446.1	hypothetical protein	-
H9210_RS05425 (1119259)	1119259..1119570	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
H9210_RS05430 (1119567)	1119567..1119986	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
H9210_RS05435 (1120100)	1120100..1121524	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
H9210_RS05440 (1121533)	1121533..1122990	-	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
H9210_RS05445 (1123250)	1123250..1124260	+	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
H9210_RS05450 (1124409)	1124409..1124936	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T171262 WP_000547564.1 NZ_CP060873:1119259-1119570 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T171262 NZ_CP077869:c1849785-1849423 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT171262 WP_000126294.1 NZ_CP060873:1119567-1119986 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT171262 NZ_CP077869:c1849952-1849782 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5