Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 662413..663212 Replicon chromosome
Accession NZ_CP060873
Organism Escherichia coli strain EC27

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag H9210_RS03210 Protein ID WP_000347251.1
Coordinates 662413..662877 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag H9210_RS03215 Protein ID WP_001307405.1
Coordinates 662877..663212 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9210_RS03180 (657414) 657414..657848 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
H9210_RS03185 (657866) 657866..658744 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
H9210_RS03190 (658734) 658734..659513 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
H9210_RS03195 (659524) 659524..659997 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
H9210_RS03200 (660020) 660020..661300 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
H9210_RS03205 (661549) 661549..662358 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
H9210_RS03210 (662413) 662413..662877 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
H9210_RS03215 (662877) 662877..663212 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
H9210_RS03220 (663361) 663361..664932 - 1572 WP_001273761.1 galactarate dehydratase -
H9210_RS03225 (665307) 665307..666641 + 1335 WP_000599652.1 galactarate/glucarate/glycerate transporter GarP -
H9210_RS03230 (666657) 666657..667427 + 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T171258 WP_000347251.1 NZ_CP060873:c662877-662413 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T171258 NZ_CP077869:c608740-608552 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATATTCGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT171258 WP_001307405.1 NZ_CP060873:c663212-662877 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT171258 NZ_CP077869:c609029-608769 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References