Detailed information of TA system TA171252

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 42456..42720
• Replicon: plasmid pEC29-3
• Accession: NZ_CP060869
• Organism: Escherichia coli strain EC29

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: H9211_RS24745
• Protein ID: WP_001331364.1
• Coordinates: 42568..42720 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	42456..42513 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9211_RS24730 (38794)	38794..39864	-	1071	WP_112041324.1	IncI1-type conjugal transfer protein TrbB	-
H9211_RS24735 (39883)	39883..41091	-	1209	WP_001383960.1	IncI1-type conjugal transfer protein TrbA	-
H9211_RS24740 (41309)	41309..42262	-	954	WP_021513958.1	hypothetical protein	-
- (42456)	42456..42513	-	58	NuclAT_0	-	Antitoxin
- (42456)	42456..42513	-	58	NuclAT_0	-	Antitoxin
- (42456)	42456..42513	-	58	NuclAT_0	-	Antitoxin
- (42456)	42456..42513	-	58	NuclAT_0	-	Antitoxin
H9211_RS24745 (42568)	42568..42720	+	153	WP_001331364.1	Hok/Gef family protein	Toxin
H9211_RS24750 (42792)	42792..43043	-	252	WP_001291964.1	hypothetical protein	-
H9211_RS25210 (43542)	43542..43637	+	96	WP_001303310.1	DinQ-like type I toxin DqlB	-
H9211_RS24760 (43702)	43702..43878	-	177	WP_001054900.1	hypothetical protein	-
H9211_RS24765 (44210)	44210..44419	+	210	WP_112041325.1	HEAT repeat domain-containing protein	-
H9211_RS24770 (44491)	44491..45153	-	663	WP_000644792.1	plasmid IncI1-type surface exclusion protein ExcA	-
H9211_RS24775 (45218)	45218..47386	-	2169	WP_077250315.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..87603	87603

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T171252 WP_001331364.1 NZ_CP060869:42568-42720 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T171252 NZ_CP077868:846778-846987 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGACGGTCAAGTAACTGTATCATATGAAACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Nucleotide

• Length: 58 bp

>AT171252 NZ_CP060869:c42513-42456 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin