Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4018300..4019027 Replicon chromosome
Accession NZ_CP060867
Organism Escherichia coli strain EC29

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag H9211_RS19410 Protein ID WP_000550189.1
Coordinates 4018713..4019027 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag H9211_RS19405 Protein ID WP_000560266.1
Coordinates 4018300..4018716 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9211_RS19395 (4013460) 4013460..4015811 + 2352 WP_000695487.1 alpha-glucosidase -
H9211_RS19400 (4016237) 4016237..4018255 + 2019 WP_000121435.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
H9211_RS19405 (4018300) 4018300..4018716 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
H9211_RS19410 (4018713) 4018713..4019027 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
H9211_RS19415 (4019311) 4019311..4020447 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
H9211_RS19420 (4020532) 4020532..4021035 + 504 WP_001333820.1 M48 family metallopeptidase -
H9211_RS19425 (4021112) 4021112..4021804 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
H9211_RS19430 (4021883) 4021883..4022869 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T171243 WP_000550189.1 NZ_CP060867:c4019027-4018713 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T171243 NZ_CP077867:1985774-1985881 [Staphylococcus aureus]
ATGTTCAATTTATTAATTGACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT171243 WP_000560266.1 NZ_CP060867:c4018716-4018300 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT171243 NZ_CP077867:c1985958-1985898 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References