171225

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 886872..887566 Replicon chromosome
Accession NZ_CP060867
Organism Escherichia coli strain EC29

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag H9211_RS04205 Protein ID WP_001263489.1
Coordinates 887168..887566 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag H9211_RS04200 Protein ID WP_000554758.1
Coordinates 886872..887165 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9211_RS04180 (882504) 882504..883001 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
H9211_RS04185 (883225) 883225..884937 - 1713 Protein_814 flagellar biosynthesis protein FlhA -
H9211_RS04190 (884909) 884909..885694 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
H9211_RS04195 (885765) 885765..886820 + 1056 WP_001226164.1 DNA polymerase IV -
H9211_RS04200 (886872) 886872..887165 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
H9211_RS04205 (887168) 887168..887566 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
H9211_RS04210 (887576) 887576..888028 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
H9211_RS04215 (888346) 888346..888552 + 207 Protein_820 RtcB family protein -
H9211_RS04220 (888548) 888548..889069 + 522 Protein_821 peptide chain release factor H -
H9211_RS04225 (889126) 889126..890583 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
H9211_RS04230 (890844) 890844..891302 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (891898) 891898..891978 + 81 NuclAT_13 - -
- (891898) 891898..891978 + 81 NuclAT_13 - -
- (891898) 891898..891978 + 81 NuclAT_13 - -
- (891898) 891898..891978 + 81 NuclAT_13 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T171225 WP_001263489.1 NZ_CP060867:887168-887566 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T171225 NZ_CP077863:1854261-1854527 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGAAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGTTATTATAGTAGACGTATCAATCACGAACATAGATTGGTCTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT171225 WP_000554758.1 NZ_CP060867:886872-887165 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT171225 NZ_CP077863:1854010-1854261 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACCTCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAATCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGAAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References