Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 880828..881369 Replicon chromosome
Accession NZ_CP060867
Organism Escherichia coli strain EC29

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag H9211_RS04165 Protein ID WP_000615983.1
Coordinates 880828..881106 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag H9211_RS04170 Protein ID WP_000729703.1
Coordinates 881109..881369 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H9211_RS04150 (878410) 878410..878988 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
H9211_RS04155 (879194) 879194..879961 + 768 WP_000333380.1 class II glutamine amidotransferase -
H9211_RS04160 (879932) 879932..880672 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
H9211_RS04165 (880828) 880828..881106 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
H9211_RS04170 (881109) 881109..881369 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
H9211_RS04175 (881579) 881579..882328 + 750 WP_000056849.1 C40 family peptidase -
H9211_RS04180 (882504) 882504..883001 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
H9211_RS04185 (883225) 883225..884937 - 1713 Protein_814 flagellar biosynthesis protein FlhA -
H9211_RS04190 (884909) 884909..885694 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T171224 WP_000615983.1 NZ_CP060867:c881106-880828 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T171224 NZ_CP077863:1829073-1829180 [Staphylococcus aureus]
ATGTTCAATTTATTAATTGACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT171224 WP_000729703.1 NZ_CP060867:c881369-881109 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT171224 NZ_CP077863:c1829257-1829197 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References