171219

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 248306..248841 Replicon plasmid pAML0157.2
Accession NZ_CP060863
Organism Enterococcus faecium strain AML0157

Toxin (Protein)


Gene name yoeB Uniprot ID R2PQC0
Locus tag IA041_RS15695 Protein ID WP_002314366.1
Coordinates 248306..248578 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q3Y220
Locus tag IA041_RS15700 Protein ID WP_002297422.1
Coordinates 248578..248841 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IA041_RS15655 243476..243925 - 450 WP_010729386.1 TlpA family protein disulfide reductase -
IA041_RS15660 244081..244623 - 543 WP_002298088.1 hypothetical protein -
IA041_RS15665 244898..245541 + 644 Protein_250 transposase -
IA041_RS15670 245617..246297 + 681 WP_002331383.1 IS6-like element IS1216 family transposase -
IA041_RS15675 246331..246480 - 150 WP_164853348.1 hypothetical protein -
IA041_RS15680 246481..247338 - 858 WP_002292680.1 AAA family ATPase -
IA041_RS15685 247623..247910 - 288 WP_002300557.1 type II toxin-antitoxin system RelE/ParE family toxin -
IA041_RS15690 247900..248229 - 330 WP_002292678.1 AbrB family transcriptional regulator -
IA041_RS15695 248306..248578 - 273 WP_002314366.1 Txe/YoeB family addiction module toxin Toxin
IA041_RS15700 248578..248841 - 264 WP_002297422.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
IA041_RS15705 249075..249983 + 909 Protein_258 IS982 family transposase -
IA041_RS15710 249986..251161 - 1176 WP_002336205.1 MFS transporter -
IA041_RS15715 251164..253413 - 2250 WP_002314387.1 alpha-galactosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..261677 261677
- inside IScluster/Tn - - 235918..259433 23515


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10780.24 Da        Isoelectric Point: 9.9724

>T171219 WP_002314366.1 NZ_CP060863:c248578-248306 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T171219 NZ_CP077861:c1040479-1040372 [Staphylococcus aureus]
ATGTTCAATTTATTAATTGACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.22 Da        Isoelectric Point: 4.5370

>AT171219 WP_002297422.1 NZ_CP060863:c248841-248578 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT171219 NZ_CP077861:1040295-1040355 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F704

Antitoxin

Source ID Structure
AlphaFold DB A0A829F006

References