Detailed information of TA system TA171155

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4488573..4489175
• Replicon: chromosome
• Accession: NZ_CP060846
• Organism: Salmonella enterica strain 733

Toxin (Protein)

• Gene name: higB
• Uniprot ID: C0Q3J8
• Locus tag: IAR32_RS21500
• Protein ID: WP_001159630.1
• Coordinates: 4488864..4489175 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IAR32_RS21495
• Protein ID: WP_000362050.1
• Coordinates: 4488573..4488863 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAR32_RS21480 (4486066)	4486066..4486968	+	903	WP_000331364.1	formate dehydrogenase subunit beta	-
IAR32_RS21485 (4486965)	4486965..4487600	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
IAR32_RS21490 (4487597)	4487597..4488526	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
IAR32_RS21495 (4488573)	4488573..4488863	-	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
IAR32_RS21500 (4488864)	4488864..4489175	-	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
IAR32_RS21505 (4489393)	4489393..4490322	+	930	WP_001127694.1	alpha/beta hydrolase	-
IAR32_RS21510 (4490408)	4490408..4490719	+	312	WP_133323219.1	type II toxin-antitoxin system HigB family toxin	-
IAR32_RS21515 (4490716)	4490716..4491162	+	447	WP_001259009.1	type II toxin-antitoxin system HigA family antitoxin	-
IAR32_RS21520 (4491177)	4491177..4492118	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
IAR32_RS21525 (4492163)	4492163..4492600	-	438	WP_000560969.1	D-aminoacyl-tRNA deacylase	-
IAR32_RS21530 (4492597)	4492597..4493469	-	873	WP_000921423.1	virulence factor BrkB family protein	-
IAR32_RS21535 (4493463)	4493463..4494062	-	600	WP_000965698.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12326.27 Da
• Isoelectric Point: 9.4460

>T171155 WP_001159630.1 NZ_CP060846:c4489175-4488864 [Salmonella enterica]
MQFIETELFTEDVKKLLDDDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGARGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T171155 NZ_CP077855:2700695-2700961 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAGGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT171155 WP_000362050.1 NZ_CP060846:c4488863-4488573 [Salmonella enterica]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT171155 NZ_CP077855:2700438-2700695 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Structures

Toxin

• AlphaFold DB: A0A0R9NS64