Detailed information of TA system TA171060

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_31
• Location: 2160021..2160697
• Replicon: chromosome
• Accession: NZ_CP060825
• Organism: Streptomyces sp. CRPJ-33

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: -
• Locus tag: IAG43_RS09505
• Protein ID: WP_187740319.1
• Coordinates: 2160021..2160383 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IAG43_RS09510
• Protein ID: WP_187740320.1
• Coordinates: 2160380..2160697 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAG43_RS09490	2155458..2157353	-	1896	WP_187740316.1	murein biosynthesis integral membrane protein MurJ	-
IAG43_RS09495	2157278..2158450	-	1173	WP_187740317.1	peptidoglycan bridge formation glycyltransferase FemA/FemB family protein	-
IAG43_RS09500	2158704..2159909	+	1206	WP_187740318.1	L,D-transpeptidase family protein	-
IAG43_RS09505	2160021..2160383	+	363	WP_187740319.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IAG43_RS09510	2160380..2160697	+	318	WP_187740320.1	helix-turn-helix domain-containing protein	Antitoxin
IAG43_RS09515	2160853..2161056	+	204	Protein_1884	XRE family transcriptional regulator	-
IAG43_RS09520	2161031..2161213	+	183	WP_187740321.1	DUF397 domain-containing protein	-
IAG43_RS09525	2161218..2162426	+	1209	WP_187740322.1	hypothetical protein	-
IAG43_RS09530	2162484..2163143	-	660	WP_187744374.1	pirin family protein	-
IAG43_RS09535	2163250..2164425	+	1176	WP_187740323.1	helix-turn-helix domain-containing protein	-
IAG43_RS09540	2164524..2165468	+	945	WP_187740324.1	ACP S-malonyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-87)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13452.06 Da
• Isoelectric Point: 4.4285

>T171060 WP_187740319.1 NZ_CP060825:2160021-2160383 [Streptomyces sp. CRPJ-33]
VLESEWEIFVVDEVRDWIDGLDGAAHARVVQALDALAEGGPGLGRPLVDTITGSALKNLKELRPGTTRILFAFDPWRSSV
LLVAGDKRDRWSEWYQEAIPLAESRYTHYLSTRTEEEGTP

Nucleotide

• Length: 363 bp

>T171060 NZ_CP077823:1865875-1865977 [Klebsiella africana]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11426.86 Da
• Isoelectric Point: 6.4865

>AT171060 WP_187740320.1 NZ_CP060825:2160380-2160697 [Streptomyces sp. CRPJ-33]
MTGYSRWKDVRAEHVERAGGEEAVAEGKRQLLAEVLGHRLAEIRASRGLTQEQVAERMGVTKGRVSHIEQGAISGQEVLI
RYATALGGELHQAIYFDNGDIAAIA

Nucleotide

• Length: 318 bp

>AT171060 NZ_CP077823:c1865979-1865839 [Klebsiella africana]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT