Detailed information of TA system TA171047

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4094087..4094676
• Replicon: chromosome
• Accession: NZ_CP060817
• Organism: Xanthomonas oryzae pv. oryzae strain C9-3

Toxin (Protein)

• Gene name: graT
• Uniprot ID: G7TIR7
• Locus tag: H9N23_RS19175
• Protein ID: WP_012443754.1
• Coordinates: 4094087..4094368 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H9N23_RS19180
• Protein ID: WP_011260746.1
• Coordinates: 4094386..4094676 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H9N23_RS19170 (H9N23_19140)	4091740..4093992	+	2253	WP_069960050.1	exodeoxyribonuclease V subunit alpha	-
H9N23_RS19175 (H9N23_19145)	4094087..4094368	+	282	WP_012443754.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H9N23_RS19180 (H9N23_19150)	4094386..4094676	+	291	WP_011260746.1	HigA family addiction module antitoxin	Antitoxin
H9N23_RS19185 (H9N23_19155)	4095605..4096999	+	1395	WP_027703652.1	type III secretion system effector XopQ	-
H9N23_RS23665	4097102..4097289	-	188	Protein_3673	hypothetical protein	-
H9N23_RS19190 (H9N23_19160)	4097449..4097781	-	333	WP_011260743.1	hypothetical protein	-
H9N23_RS19195 (H9N23_19165)	4098036..4098362	+	327	WP_228329596.1	hypothetical protein	-
H9N23_RS19200 (H9N23_19170)	4098499..4098912	+	414	WP_228383576.1	PAAR domain-containing protein	-
H9N23_RS19205 (H9N23_19175)	4098915..4099523	+	609	WP_113195694.1	DUF4123 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11066.83 Da
• Isoelectric Point: 10.5318

>T171047 WP_012443754.1 NZ_CP060817:4094087-4094368 [Xanthomonas oryzae pv. oryzae]
VIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T171047 NZ_CP077820:405110-405394 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT171047 WP_011260746.1 NZ_CP060817:4094386-4094676 [Xanthomonas oryzae pv. oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT171047 NZ_CP077820:404878-405120 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A0K0GFW5